- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Yeast (S. cerevisiae), H, R, M, B, Rb, Xn WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-421 品牌系列 Upstate
商名 - Upstate
描述 Anti-PP2A Antibody, C subunit, clone 1D6 Alternate Names - serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform
- Replication protein C
- protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
- protein phosphatase 2, catalytic subunit, alpha isoform
- alpha isoform
- protein phosphatase 2
背景信息 Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine. 产品信息 格式 Purified 控制 - HeLa whole cell lysate, mouse NIH3T3 cells, A431 cell lysate.
Included Positive Antigen Control: Catalog # 12-301, non stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15 M, NaCl, and 0.05% sodium azide. Frozen solution. 应用 应用 Detect PP2A using this Anti-PP2A Antibody, C subunit, clone 1D6 validated for use in IC, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunoprecipitation:
4 µg of a previous lot immunoprecipitated PP2A from 500 µg of A431 RIPA lysate. Note: This antibody has been reported not to block phosphatase activity.
Immunocytochemistry:
5-10 µg/mL of a previous lot of this antibody showed positive immunostaining for PP2A in A431 cells fixed for 10 minutes with 4% paraformaldehyde in PBS.生物信息 免疫原品种 A 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A). Clone 1D6. 表位 a.a. 295-309 克隆 1D6 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes PP2A catalytic subunit, Mr 36 kDa. 同种型 IgG2bκ 品种反应性 Yeast (S. cerevisiae)HumanRatMouseBovineRabbitXenopus Species Reactivity Note Human, bovine, mouse, rat, rabbit, Xenopus and yeast. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002706
Entrez基因汇总 This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. 基因符号 - RP-C
- PPP2CA
- PP2CA
- PP2A-alpha
- PP2Ac
纯化方法 Protein A chromatography UniProt编号 - P67775
UniProt汇总 FUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
SIZE: 309 amino acids; 35594 Da
SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.产品使用声明 质量保证 Routinely evaluated by western blot on human A431 or murine 3T3/NIH RIPA cell lysates.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected PP2A in human A431 and previously in murine 3T3 RIPA cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,421050,(Z-LL)₂ Ketone - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,ELI-008-H,IL-6 ELISPOT Antibody Pair, Human
相关产品
merck millipore,默克密理博,09-724,Anti-RNF110/PCGF2 Antibody
merck millipore,默克密理博,196878,(±)-Bay K 8644 - CAS 93468-89-4 - Calbiochem
merck millipore,默克密理博,AB2083,Anti-CD44v7 Antibody, exon v7
merck millipore,默克密理博,12-645,mTOR Substrate
merck millipore,默克密理博,421050,(Z-LL)₂ Ketone - Calbiochem
merck millipore,默克密理博,ELI-008-H,IL-6 ELISPOT Antibody Pair, Human
merck millipore,默克密理博,MZHVN0W50,MultiScreenHTS HV，0.45 µm，白色，非无菌
merck millipore,默克密理博,341668,Fibronectin, Rat Plasma
merck millipore,默克密理博,MABC696,Anti-EPHX2, clone 60083-1 Antibody
merck millipore,默克密理博,236020,Etomoxir - CAS 828934-41-4 - Calbiochem