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merck millipore,默克密理博,05-312,Anti-E2F-4 Antibody, clone GG22-2A6
产品名称：Anti-E2F-4 Antibody, clone GG22-2A6
产品型号：05-312
Detect E2F-4 with Anti-E2F-4 Antibody, clone GG22-2A6 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
merck millipore,默克密理博,05-312,Anti-E2F-4 Antibody, clone GG22-2A6
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB M Purified Monoclonal Antibody 描述 产品目录编号 05-312 品牌系列 Upstate
商名 - Upstate
描述 Anti-E2F-4 Antibody, clone GG22-2A6 产品信息 格式 Purified 演示 200μg of protein G purified mouse IgG2b in 200μl of 0.07 M Tris-glycine, pH 7.4, 0.135 M NaCl, 0.035% sodium azide and 30% glycerol. 应用 应用 Detect E2F-4 with Anti-E2F-4 Antibody, clone GG22-2A6 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 GST fusion to human E2F-4 克隆 clone GG22-2A6 宿主 Mouse 特异性 E2F-4; does not recognize E2F-1, -2, -3, or -5 同种型 IgG2b 品种反应性 RatHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001950
Entrez基因汇总 The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. 基因符号 - E2F-4
- E2F4
纯化方法 Protein G chromatography UniProt编号 - Q16254
UniProt汇总 FUNCTION: SwissProt: Q16254 # Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
SIZE: 413 amino acids; 43960 Da
SUBUNIT: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. The E2F4/DP-1 dimer interacts preferentially with pocket protein RBL1, which inhibits the E2F transactivation domain. Lower affinity interaction has been found with retinoblastoma protein RB1. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Interacts with HCFC1. Component of the DREAM complex composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBBP4, RBL2, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.DEVELOPMENTAL STAGE: Present in the growth-arrested state, its abundance does not change significantly as cells move into and through the cell cycle.
PTM: Differentially phosphorylated in vivo.
SIMILARITY: SwissProt: Q16254 ## Belongs to the E2F/DP family.产品使用声明 质量保证 routinely evaluated on non-stimulated A431 cell lysate 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -20°C 包装信息 数量 200 µg
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