merck millipore,默克密理愽
  • merck millipore,默克密理博,05-217,Anti-PI3 Kinase Antibody, p85, N-SH2,

  • merck millipore,默克密理博,05-217,Anti-PI3 Kinase Antibody, p85, N-SH2,

    产品名称：Anti-PI3 Kinase Antibody, p85, N-SH2,
    产品型号：05-217
    Anti-PI3 Kinase Antibody, p85, N-SH2, is an antibody against PI3 Kinase for use in WB & IP.

    merck millipore,默克密理博,05-217,Anti-PI3 Kinase Antibody, p85, N-SH2,

  • 产品介绍
  • merck millipore,默克密理博,05-217,Anti-PI3 Kinase Antibody, p85, N-SH2,

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MWBMCulture SupernatantMonoclonal Antibody
    描述
    产品目录编号 05-217
    Replaces 04-403
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-PI3 Kinase Antibody, p85, N-SH2,
    Alternate Names
    • phosphoinositide-3-kinase, catalytic, alpha polypeptide
    • PI3-kinase p110 subunit alpha
    • PtdIns-3-kinase p110
    • phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha
    • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
    • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic
    • subunit, alpha isoform
    • alpha polypeptide
    • phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic
    • subunit
    • alpha isoform
    背景信息 The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that is thought to phosphorylate second messengers in growth signalling pathways. PI3K exists as a heterodimer of catalytic 110 kDa (p110) and regulatory 85 kDa (p85, p50 or p55) subunits. The p85 unit consists of two closely related proteins: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. They share 62% amino acid sequence identity. p85 alpha lacks PI3 kinase activity, but may serve as regulator of the catalytic subunit, p110, by acting as the link between PI3K and the ligand activated receptor. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.
    产品信息
    格式 Culture Supernatant
    控制
    • Positive Control Included: Non-Stim A431 Cell Lysate (12-301).
      Included Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
    演示 Mouse monoclonal IgG in buffer containing culture supernatant fraction in frozen solution.
    应用
    应用 Anti-PI3 Kinase Antibody, p85, N-SH2, is an antibody against PI3 Kinase for use in WB & IP.
    主要应用
    • Western Blotting
    应用说明 Immunoprecipitation: Use 5 µL per sample.
    生物信息
    免疫原品种 GST fusion protein corresponding to N-Terminal SH2 region (amino acid residues 327-434) of rat phosphoinositide 3-Kinase.
    表位 N-terminus
    克隆 clone UB93-3
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Recognizes N-SH2 region of p85 alpha subunit of PI3 Kinase.
    同种型 IgG
    品种反应性 RatHumanMouse
    Species Reactivity Note Human, rat; mouse; other species cross-reactivity is unknown.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_852556
    Entrez基因汇总 Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms.
    基因符号
    • p85-ALPHA
    • PIK3R1
    • GRB1
    • PI3K
    纯化方法 Unpurified
    UniProt编号
    • P27986
    UniProt汇总 FUNCTION: SwissProt: P27986 # Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
    SIZE: 724 amino acids; 83598 Da
    SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C- terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1.
    TISSUE SPECIFICITY: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).
    DOMAIN: SwissProt: P27986 The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.
    PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.DISEASE:SwissProt: P27986 # Defects in PIK3R1 are a cause of severe insulin resistance.
    SIMILARITY: Belongs to the PI3K p85 subunit family. & Contains 1 Rho-GAP domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain.
    产品使用声明
    质量保证 Western blot Analysis: A 1:100-1:200 dilution of this antibody detected PI3-Kinase in RIPA lysates from human A431 carcinoma cells and previously from mouse 3T3/A31 fibroblasts.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at from date of receipt.
    包装信息
    数量 200 µL

    merck millipore,默克密理博,05-217,Anti-PI3 Kinase Antibody, p85, N-SH2,

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