merck millipore,默克密理博,04-1062,Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1062,Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal
产品名称：Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal
产品型号：04-1062
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect GFAP (N-term) using this Anti-GFAP (N-term) A More>>

merck millipore,默克密理博,04-1062,Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1062,Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1062
描述	Anti-GFAP (NT) Antibody, clone EPR1034Y, rabbit monoclonal
Alternate Names	glial fibrillary acidic protein
背景信息	Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

产品信息
格式	Unpurified
控制	Human and Rat brain lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect GFAP (N-term) using this Anti-GFAP (N-term) Antibody, clone EPR1034Y, Rabbit validated for use in WB, IH(P), IC & IP.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected GFAP in human brain and human glioma tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC. Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in the N-terminus of human GFAP.
表位	N-terminus
克隆	EPR1034Y
宿主	Rabbit
特异性	This antibody recognizes GFAP at and around the N-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001124491.1
Entrez基因汇总	This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
基因符号	FLJ45472
纯化方法	Unpurified
UniProt编号	P14136
UniProt汇总	FUNCTION: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. SUBUNIT STRUCTURE: Interacts with SYNM By similarity. Isoform 3 interacts with PSEN1 (via N-terminus). SUBCELLULAR LOCATION: Cytoplasm. Note: Associated with intermediate filaments. TISSUE SPECIFICITY: Expressed in cells lacking fibronectin. INVOLVEMENT IN DISEASE: Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. SEQUENCE SIMILARITIES: Belongs to the intermediate filament family.

产品使用声明
质量保证	Evaluated by Western Blot on Human and Rat brain cell lysates. Western Blot Analysis: 1:50,000-100,000 dilution of this antibody was used to detect GFAP in human and rat brain cell lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.