merck millipore,默克密理博,17-10246,ChIPAb+™ Histone H2A.Bbd

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merck millipore,默克密理博,17-10246,ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set
产品名称：ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set
产品型号：17-10246
This ChIPAb+ Histone H2A.Bbd -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,17-10246,ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,17-10246,ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
M, H	ChIP, EMSA, IHC, WB

描述
产品目录编号	17-10246
商名	Upstate ChIPAb+
描述	ChIPAb+™ Histone H2A.Bbd - ChIP Validated Antibody and Primer Set
概述	All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Histone H2A.Bbd set includes the Histone H2A.Bbd antibody, a Normal Rabbit IgG, and control primers which amplify a 70 bp region of ChIP primers, PGK1. The Histone H2A.Bbd and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H2A.Bbd -associated chromatin.
Alternate Names	histone H2A-Bbd type 1 H2A histone family, member B1 H2A Barr body-deficient
背景信息	Histone H2A.Bbd (Barr body-deficient), also known as histone H2A-Bbd type 1, is an atypical histone variant. This histone variant associates with H2B and replaces histone H2A in the nucleosome. In contrast to other H2A histones, this variant lacks conserved residues that are targets of post-translational modifications. Consequently this H2A.Bbd variant is about 15 amino acids shorter and only 48% similar to the canonical H2A. The major differences between the two are found in the docking domain that links H2A -H2B to the H3-H4 tetramer. Nucleosomes containing this histone adopt a more relaxed structure and protect ~118 base pairs of DNA instead of the 147 base pairs found in classical nucleosomes. This variant histone is associated with active X chromosomes and autosomes, and is typically absent from inactive X chromosomes.

产品信息
格式	Affinity Purified
控制	Includes normal rabbit IgG and primers specific for human PGK1.
演示	Anti-Histone H2A.Bbd (rabbit polyclonal). One vial containing 100 µg of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C Concentration: 0.7 mg/mL Normal Rabbit IgG, . One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C. ChIP primers, PGK1. One vial containing 75 μL of 5 μM of each primer specific for human PGK1. Store at -20°C. FOR: CCT TCC CCC ACG CTC CTA REV: TTG TGC AGG AAC CTG TGA GAT G

应用
应用	This ChIPAb+ Histone H2A.Bbd -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Chromatin Immunoprecipitation (ChIP) Electrophoretic Mobility Shift Assay Immunohistochemistry Western Blotting
应用说明	Western Blot Analysis: Representative lot data. H2A.Bbd overexpression (Lane 1-2), various recombinant Histone proteins (Lane 4-7) and HeLa acid extract (Lane 8) lysates were probed with Anti-Histone H2A.Bbd (0.5 µg/mL); Lane 1: 500 ng of H2A.Bbd overexpression lysate Lane 2: 1 µg H2A.Bbd overexpression lysate Lane 3: 500 ng Vector transfected cell lysate control Lane 4: 500 ng Recombinant Histone H2A (Cat.#: 14-493) Lane 5: 500 ng Recombinant Histone H2B (Cat.#: 14-491) Lane 6: 500 ng Recombinant Histone H3 (Cat.#: 14-494) Lane 7: 500 ng Recombinant Histone H4 (Cat.#: 14-697) Lane 8: 10 µg HeLa acid extract Proteins were visualized using a Donkey Anti Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H2A.Bbd (~17 kDa). (Figure 2).

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human Histone H2A.Bbd.
表位	a.a. 35-55
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes human Histone H2A.Bbd.
品种反应性	MouseHuman
Species Reactivity Note	Demonstrated to react with human and mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_001017990
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the most centromeric copy which is in intron 22 of the F8 gene. [provided by RefSeq].
基因符号	H2AFB1 H2A.Bbd
纯化方法	Affinity purified
UniProt编号	P0C5Y9
UniProt汇总	FUNCTION: Atypical histone H2A which can replace conventional H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Nucleosomes containing this histone are less rigid and organize only 118 base pair of DNA instead of 147 in classical nucleosomes. They are associated with transcriptionally active chromatin and excluded form Barr bodies. Ref.4 Ref.5 SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. May be incorporated into a proportion of nucleosomes, replacing one or more H2A molecules. SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies. DOMAIN: The docking domain is responsible for the weaker heterodimerization with H2B. Ref.4 MISCELLANEOUS: In contrast to other H2A histones, it does not contain the conserved residues that are the target of post-translational modifications. SEQUENCE SIMILARITIES: Belongs to the histone H2A family.

产品使用声明
质量保证	Chromatin Immunoprecipitation: Representative lot data. Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either Normal Rabbit IgG, or 4 µg of Anti-Histone H2A.Bbd, and the buffers from the EZ-Magna ChIP™ A Kit (Cat. # 17-408) plus the 16-663 Magna ChIP Protein A+G Beads. Successful immunoprecipitation of Histone H2A.Bbd associated DNA fragments was verified by qPCR using ChIP primers specific for the human PGK1 region as a positive locus (Figure 1).
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Chromatin Immunoprecipitation:
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either Normal Rabbit IgG, or 4 µg of Anti-Histone H2A.Bbd, and the buffers from the EZ-Magna ChIP™ A Kit (Cat. # 17-408) plus the 16-663 Magna ChIP Protein A+G Beads. Successful immunoprecipitation of Histone H2A.Bbd associated DNA fragments was verified by qPCR using ChIP primers specific for the human PGK1 region as a positive locus (Figure 1).

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.