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品种反应性 主要应用 宿主 格式 抗体类型 Rb, H, B, M, R WB M Purified Monoclonal Antibody 描述 产品目录编号 05-154 品牌系列 Upstate
商名 - Upstate
描述 Anti-PKCα Antibody, clone M4 Alternate Names - protein kinase C, alpha
- aging-associated gene 6
- protein kinase C alpha type
- protein kinase C
背景信息 Protein Kinase c alpha (PKC alpha) is a 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell-cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy-terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation. 产品信息 格式 Purified 控制 - Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
演示 Purified mouse monoclonal IgG1 in storage buffer containing (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). 应用 应用 Anti-PKCα Antibody, clone M4 is an antibody against PKCα for use in IP, NEUT, RIA & WB. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
4 μg of a previous lot immunoprecipitated PKCα from an A431 RIPA cell lysate and a bovine brain extract.
Neutralization:
An independent laboratory showed that 20 μg of this antibody can inhibit >50% of the kinase activity of 20 ng PKCα (Leach, K.L., 1998).生物信息 免疫原品种 PKCα purified from rabbit brain cytosol. 克隆 M4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Specific for PKCα. MW: ~82 kDa. 同种型 IgG1 品种反应性 RabbitHumanBovineMouseRat Species Reactivity Note Bovine, human, mouse, rat and rabbit. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002737
Entrez基因汇总 Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. 基因符号 - PKCA
- PRKCA
- PKC-A
- AAG6
- PKC-alpha
- MGC129901
- MGC129900
- PRKACA
纯化方法 Protein G purfied UniProt编号 - P17252
UniProt汇总 FUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
SIZE: 672 amino acids; 76764 Da
SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated in A431 RIPA cell lysate or bovine brain cytosolic extract.
Western Blot Analysis:
0.5-2 μg/mL of this antibody detected PKCα from a human A431 RIPA cell lysate. Previous lots detected PKCα in bovine brain cytosolic extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,ST1672,Anti-DPYD Mouse mAb (7D4)
下一件merck millipore产品：merck millipore,默克密理博,17-10521,EZ-Magna Nuclear RIP™ (Cross-Linked)Nuclear RNA-Binding Protein Immunoprecipitation Kit
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