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merck millipore,默克密理博,05-1047,Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2
产品名称：Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2
产品型号：05-1047
Detect EGFR (cytoplasmic domain) using this Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2 validated for use in IC, IH, IP & WB.
merck millipore,默克密理博,05-1047,Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 05-1047 Replaces 04-338 描述 Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2 Alternate Names - epidermal growth factor receptor (erythroblastic leukemia<br />viral (v-erb-b) oncogene homolog, avian)
- Receptor tyrosine-protein kinase ErbB-1
- avian erythroblastic leukemia viral (v-erb-b) oncogene<br />homolog
- cell growth inhibiting protein 40
- cell proliferation-inducing protein 61
- epidermal growth factor receptor
- epidermal growth factor receptor (avian erythroblastic<br />leukemia viral (v-erb-b) oncogene homolog)
- epidermal growth factor receptor (erythroblastic leukemia<br />viral (v-erb-b) oncogene homolog
背景信息 The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFα). Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer. In addition to forming homodimers after ligand binding, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. There is also evidence to suggest that clusters of activated EGFRs form, although it remains unclear whether this clustering is important for activation itself or occurs subsequent to activation of individual dimers. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine (Y) residues in the C-terminal domain of EGFR occurs. These are Y992, Y1045, Y1068, Y1148 and Y1173. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding SH2 domains. These downstream signaling proteins initiate several signal transduction cascades, principally the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. Such proteins modulate phenotypes such as cell migration, adhesion, and proliferation. The kinase domain of EGFR can also cross-phosphorylate tyrosine residues of other receptors it is aggregated with, and can itself be activated in that manner. 产品信息 格式 Purified 演示 Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect EGFR (cytoplasmic domain) using this Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2 validated for use in IC, IH, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunocytochemistry Analysis:
A431 cells were grown, fixed, permeablized, and stained with anti-EGFR, clone 8G6.2
Confocal Immunocytochemistry Analysis:
A431 cells were grown, fixed, permeablized, and stained with anti-EGFR, clone 8G6.2
Immunoprecipitation:
100 μg of A431 whole cell lysate was lysed in RIPA buffer and immunoprecipitated (IP) with 5 μg of Anti-EGFR, cytoplasmic domain, clone 8G6.2
Immunohistochemistry Analysis:
Tissue was stained with anti-EGFR, cytoplasmic domain, clone 8G6.2 at a 1:100 dilution and IHC-Select Detection with HRP-DAB reagents.
生物信息 免疫原品种 Recombinant protein encoding the cytoplasmic domain (C-terminal) of human EGF Receptor. 表位 Cytoplasmic Domain 克隆 8G6.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody detects the cytoplasmic domain of EGFR. 同种型 IgG1κ 品种反应性 RatHumanMouse Species Reactivity Note Evaluated by western blot on A431 cell lysate
Western Blot Analysis:
1:2,000 dilution of this antibody was used to detect EGFR in A431 cell lysate.抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005219
Entrez基因汇总 The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
基因符号 - mENA
- EGFR
- ERBB1
- ERBB
纯化方法 Protein G purfied UniProt编号 - P00533
UniProt汇总 FUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
SIZE: 1210 amino acids; 134277 Da
SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.产品使用声明 质量保证 Evaluated by western blot on A431 cell lysate
Western Blot Analysis:
1:2,000 dilution of this antibody was used to detect EGFR in A431 cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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