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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 04-903 Replaces 05-903 描述 Anti-GSK3α/β Antibody, rabbit monoclonal Alternate Names - glycogen synthase kinase 3 alpha
- GSK-3 alpha
背景信息 Glycogen Synthase Kinase 3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway. 产品信息 格式 Culture Supernatant 控制 - HEK293 cell lysate
演示 Cultured supernantant containing 0.05% sodium azide. 应用 应用 This Anti-GSK3α/β Antibody is validated for use in WB, IP for the detection of GSK3α/β. 主要应用 - Western Blotting
应用说明 Immunoprecipitation: 2-8 μL of a previous lot of antibody immunoprecipitated GSK3 alpha and beta from 100 μg of HEK293 RIPA lysate.
NOTE: This antibody also successfully immunoprecipitated GSK3 alpha and beta using the Catch and Release® v2.0 Reversible Immunoprecipitation System (Catalog # 17-500).
生物信息 免疫原品种 N-terminal 6His-tagged recombinant full length human GSK3β. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 GSK3 alpha and beta. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human and mouse.
Predicted to cross-react with rat based on sequence homology.抗体类型 Monoclonal Antibody Entrez基因编号 - NP_063937.2, NP_002084.2
Entrez基因汇总 Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase,
homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of
several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription
factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)基因符号 - EC 2.7.11.26
- DKFZp686D0638
UniProt编号 - P49840
UniProt汇总 FUNCTION:Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN By similarity.
CATALYTIC ACTIVITY: ATP + [tau protein] = ADP + [tau protein] phosphate.
SUBUNIT STRUCTURE:Monomer By similarity.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
产品使用声明 质量保证 Evaluated by western blot on lysates from HEK293 cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSK3 alpha and GSK3 beta in RIPA lysates from A431, HEK293, Jurkat, HeLa NE or 3T3/A31 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µl
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