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merck millipore,默克密理博,04-820,Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal
产品名称：Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal
产品型号：04-820
Anti-Estrogen Receptor α Antibody, clone 60C is an antibody against Estrogen Receptor α for use in WB & IP.
merck millipore,默克密理博,04-820,Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal
- 产品介绍
Replacement Information Replacement Information 04-820 is a recommended replacement for 05-820 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Po, H, M, R, B WB Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 04-820 Replaces 05-820 描述 Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal Alternate Names - steroid hormone receptor
- Estradiol receptor
- Nuclear receptor subfamily 3 group A member 1
- estrogen receptor 1
- estrogen receptor alpha
- oestrogen receptor
背景信息 The Estrogen Receptors (ER) belong to the nuclear receptor family that contains a ligand-binding domain that maintains the protein in an inactive conformation unless bound to the hormone. Two ERs are known, ERα, classically known as ER, and ERβ, described long after ERα. Activated Estrogen Receptor is a DNA-binding protein capable of inducing many changes in gene expression. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα. 产品信息 格式 Culture Supernatant 演示 Cultured supernantant with 0.05% sodium azide. 应用 应用 Anti-Estrogen Receptor α Antibody, clone 60C is an antibody against Estrogen Receptor α for use in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
4-10 μL of a previous lot of this clone immunoprecipitated Estrogen Receptor α from 1 mg of MCF-7 RIPA lysate.
生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to amino acids 13-32 (C-ALLHQIQGNELEPLNRPQLK) of human Estrogen Receptor α. The immunizing sequence is identical in rat and mouse and has 19/20 identical amino acids in bovine and porcine. 表位 N-term 克隆 60C 宿主 Rabbit 特异性 Recognizes Estrogen Receptor α. No cross-reactivity with Estrogen Receptor β. 同种型 IgG 品种反应性 PigHumanMouseRatBovine Species Reactivity Note Human. Predicted to cross-react with rat, mouse, bovine and porcine based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000116.2
Entrez基因汇总 Summary: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for
hormone binding, DNA binding, and activation of transcription. The
protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast
cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters. [provided by RefSeq].
基因符号 - OTTHUMP00000017719
- ER
- ER-alpha
- ESR
- ESRA
- Era
- NR3A1
- DKFZp686N23123
- OTTHUMP00000017718
纯化方法 Unpurified UniProt编号 - P03372
UniProt汇总 FUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
SIZE: 595 amino acids; 66216 Da
SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.产品使用声明 质量保证 Routinely evaluated by western blot analysis.
Western Blot Analysis:
This lot detected ERα at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
包装信息 数量 100 µL
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下一件merck millipore产品：merck millipore,默克密理博,400122,Human IgG₂, Myeloma
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