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merck millipore,默克密理博,ABC124,Anti-phospho-ULK1 (Ser555) Antibody
产品名称：Anti-phospho-ULK1 (Ser555) Antibody
产品型号：ABC124
This Anti-phospho-ULK1 (Ser555) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay for the detection of phospho-ULK1 (Ser555).
merck millipore,默克密理博,ABC124,Anti-phospho-ULK1 (Ser555) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Xn, H, M, R, B WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABC124 描述 Anti-phospho-ULK1 (Ser555) Antibody Alternate Names - Unc-51-like kinase 1
- Serine/threonine-protein kinase ULK1
- Autophagy-related protein 1 homolog
- ATG1
- hATG1
背景信息 ULK1 or autophagy-related protein 1 homolog (ATG1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1's enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins. 产品信息 格式 Affinity Purified 控制 - Non-serum starved and serum starved MCF7 cell lysates
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-phospho-ULK1 (Ser555) Antibody is validated for use in Western Blotting, Peptide Inhibition Assay for the detection of phospho-ULK1 (Ser555). 主要应用 - Western Blotting
应用说明 Peptide Inhibition Analysis: A representative lot of this antibody blocked ULK1 in PIA involving peptide 3123-C vs. peptide 3123 on serum starved MCF7 cell lysates. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human ULK1 phosphorylated at Ser555. 表位 Phosphorylated Ser555 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes ULK1 phosphorylated at Ser555. 品种反应性 XenopusHumanMouseRatBovine Species Reactivity Note Demonstrated to react with Human. Predicted to react with Mouse, Rat, Bovine, and Xenopus based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003556
基因符号 - ULK1
修改 - Phosphorylation
纯化方法 Affinity purified UniProt编号 - O75385
UniProt汇总 FUNCTION: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SUBUNIT STRUCTURE: ATP + a protein = ADP + a phosphoprotein.
SUBCELLULAR LOCATION: Cytoplasm > cytosol. Preautophagosomal structure. Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
TISSUE SPECIFICITY: Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
POST-TRANSLATIONAL MODIFICATION: Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1. In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.
SEQUENCE CAUTION: The sequence BAA34442.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.产品使用声明 质量保证 Evaluated by Western Blot in non-serum starved and serum starved MCF7 cell lysates.
Western Blot Analysis: 0.2 µg/mL of this antibody detected phospho-ULK1 (Ser555) in 10 µg of non-serum starved and serum starved MCF7 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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