- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Monoclonal Antibody 描述 产品目录编号 04-784 Replaces 05-784 品牌系列 Upstate
商名 - Upstate
描述 Anti-IRS1 Antibody, clone AW58 | 04-784 Alternate Names - insulin receptor substrate 1
背景信息 Insulin Receptor substrate proteins are the effectors of both Insulin and IGF-initiated signaling. They share PH and PTB domains near their n-termini, and multiple Tyr phosphorylation motifs in their c-terminal regions. Proteins which bind to tyrosine-phosphorylated IRS-proteins include PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. IRS1 appears to be principally involved in IGF-signaling and cytoskeletal growth. IRS2 appears to be the critical mediator of Insulin signaling, as genetic ablation results in type II diabetes. IRS3 is expressed primarily in adipocytes and is an unusually potent activator of PI3 Kinase. IRS4 lacks the tyrosine residues which in the other IRS-proteins binds to SHP2. 产品信息 格式 Purified 演示 Cultured supernantant in 0.05% sodium azide 应用 主要应用 - Western Blotting
应用说明 Immunoblot Analysis: 1:1,000 to 1:4,000 dilutions of this lot detected IRS1 in RIPA lysates from 3T3/A31 mouse fibroblasts.
Immunoprecipitation: 5-10μl of this lot immunoprecipitated IRS1 from 500μg of 3T3/A31 RIPA lysate.
生物信息 免疫原品种 Peptide corresponding to carboxy-terminal 14 amino acids (C-YASINFQKQPEDRQ) of rat liver IRS1 克隆 AW58 宿主 Rabbit 特异性 IRS1 同种型 IgG 品种反应性 RatHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005544
基因符号 - HIRS-1
- IRS1
- IRS-1
UniProt编号 - P35568
UniProt汇总 FUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
SIZE: 1242 amino acids; 131591 Da
SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain.产品使用声明 质量保证 routinely evaluated by immunoblot on in RIPA lysates from 3T3/A31 mouse fibroblasts 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at -20°C from date of shipment 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,04-1134,Anti-Estrogen Related Receptor α Antibody, clone EPR46Y, rabbit monoclonal
下一件merck millipore产品：merck millipore,默克密理博,AB8126,Anti-phospho-Integrin β1 (Tyr795) Antibody
相关产品
merck millipore,默克密理博,MAB5622,Anti-Secretagogin Antibody
merck millipore,默克密理博,AB133,Anti-Glutamate Antibody
merck millipore,默克密理博,119102,Anti-H+,K+-ATPase, α-Subunit (1-17) Rabbit pAb
merck millipore,默克密理博,AB8126,Anti-phospho-Integrin β1 (Tyr795) Antibody
merck millipore,默克密理博,MAB8184-K,Anti-EBV VCA Gp125, Clone L2
merck millipore,默克密理博,MAB1503,Anti-Nogo A Antibody, clone 6A5.2
merck millipore,默克密理博,PP031-KC,Bovine IgG (Gamma Globulin) (KC)