merck millipore,默克密理博,04-1134,Anti-Estrogen Related Receptor α Antibody, clone EPR46Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1134,Anti-Estrogen Related Receptor α Antibody, clone EPR46Y, rabbit monoclonal
产品名称：Anti-Estrogen Related Receptor α Antibody, clone EPR46Y, rabbit monoclonal
产品型号：04-1134
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Estrogen Related Receptor α using this Anti-E More>>

merck millipore,默克密理博,04-1134,Anti-Estrogen Related Receptor α Antibody, clone EPR46Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1134,Anti-Estrogen Related Receptor α Antibody, clone EPR46Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1134
描述	Anti-Estrogen Related Receptor α Antibody, clone EPR46Y, rabbit monoclonal
Alternate Names	estrogen-related receptor alpha Estrogen receptor-like 1 Estrogen-related receptor, alpha Nuclear receptor subfamily 3 group B member 1
背景信息	This protein is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. In mouse, ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in mouse in brain, spinal cord, pituitary gland and heart.

产品信息
格式	Unpurified
控制	HeLa cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect Estrogen Related Receptor α using this Anti-Estrogen Related Receptor α Antibody, clone EPR46Y, Rabbit validated for use in WB, IP, FC.
主要应用	Flow Cytometry Western Blotting
应用说明	Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry. Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminus of human Estrogen Related Receptor α.
表位	N-terminus
克隆	EPR46Y
宿主	Rabbit
特异性	This antibody recognizes Estrogen Related Receptor α.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_004442.3
基因符号	NR3B1 ERR-alpha ERR1 ERRa ERRalpha ESRL1
纯化方法	Unpurified
UniProt编号	P11474
UniProt汇总	FUNCTION: Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. SUBUNIT STURCTURE: Binds DNA as a monomer or a homodimer. Interacts (via the AF2 domain) with coactivator PPARGC1A (via the L3 motif); the interaction greatly enhances transriptional activity of genes involved in energy metabolism. Interacts with PIAS4; the interaction enhances sumoylation. SUBCELLULAR LOCATION: Nucleus INDUCTION: Induced by PGC1alpha in a number of specific cell types including heart, kidney and muscle. PTM: Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity. Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding. SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain. SEQUENCE CAUTION: The sequence CAA35778.1 differs from that shown. Reason: Frameshift at positions 345 and 354.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa cell lysates. Western Blot Analysis: A 1:2,500-5,000 dilution of this antibody was used to detect Estrogen Related Receptor α in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.