merck millipore,默克密理博,04-467,Anti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal

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merck millipore,默克密理博,04-467,Anti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal
产品名称：Anti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal
产品型号：04-467
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-LAT (Tyr191) using this Anti-phospho- More>>

merck millipore,默克密理博,04-467,Anti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-467,Anti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-467
品牌系列	Upstate
商名	Upstate
描述	Anti-phospho-LAT (Tyr191) Antibody, rabbit monoclonal

产品信息
格式	Purified
演示	100 μL of rabbit monoclonal IgG in 60% storage buffer (50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect phospho-LAT (Tyr191) using this Anti-phospho-LAT (Tyr191) Antibody, Rabbit validated for use in WB, IH(P).
主要应用	Western Blotting

生物信息
免疫原品种	KLH-conjugated synthetic phosphor-peptide encompassing residues surrounding Tyrosine 191 of human LAT.
宿主	Rabbit
特异性	Recognizes a synthetic phosphor-peptide encompassing residues surrounding Tyrosine 191 of human LAT. This antibody will react only with LAT phosphorylated at Tyrosine 191.
同种型	IgG
品种反应性	RatHumanMouse
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_014387.3 NM_001014987.1 NM_001014988.1 NM_001014989.1
Entrez基因汇总	The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
基因符号	p36-38 LAT pp36 LAT1
修改	Phosphorylation
UniProt编号	O43561
UniProt汇总	FUNCTION: SwissProt: O43561 # Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. SIZE: 262 amino acids; 27930 Da SUBUNIT: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB and SKAP2. SUBCELLULAR LOCATION: Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts. TISSUE SPECIFICITY: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). PTM: Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. & Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. MISCELLANEOUS: Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell- induced activation of PLC, maybe by inducing the dephosphorylation of LAT.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.