merck millipore,默克密理博,04-399,Anti-PI3 Kinase Antibody, p110α rabbit monoclonal

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merck millipore,默克密理博,04-399,Anti-PI3 Kinase Antibody, p110α rabbit monoclonal
产品名称：Anti-PI3 Kinase Antibody, p110α rabbit monoclonal
产品型号：04-399
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PI3 Kinase Antibody, p110α Rabbit detects level More>>

merck millipore,默克密理博,04-399,Anti-PI3 Kinase Antibody, p110α rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-399,Anti-PI3 Kinase Antibody, p110α rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-399
品牌系列	Upstate
商名	Upstate
描述	Anti-PI3 Kinase Antibody, p110α rabbit monoclonal
Alternate Names	phosphoinositide-3-kinase, catalytic, alpha polypeptide PI3-kinase p110 subunit alpha PtdIns-3-kinase p110 phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha phosphatidylinositol 3-kinase, catalytic, alpha polypeptide phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,<br />alpha isoform alpha alpha polypeptide phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit,<br />alpha isoform
背景信息	PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

产品信息
格式	Purified
控制	Jurkat cell lysate
演示	Purified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide, 0.05% BSA and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PI3 Kinase Antibody, p110α Rabbit detects level of PI3 Kinase & has been published & validated for use in IF, IP & WB.
主要应用	Western Blotting
应用说明	Immunoprecipitation: A 1:30 dilution of a previous lot immunoprecipitated PI3 Kinase, p110a using protein G agarose beads. Immunfluorescence Staining: 1:100-1:250 dilution of a previous lot was used for Immunfluorescence staining of paraformaldehyde fixed HeLa cells.

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PI3 Kinase, p110α
表位	C-terminus
宿主	Rabbit
特异性	This antibody recognizes the C-terminal domain of PI3 Kinase, p110α.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human. Reactivity with other species has not been confirmed. Not believed to cross-react with mouse or rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_006218.2
Entrez基因汇总	Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
基因符号	MGC142163 PIK3CA MGC142161 PI3K p110-alpha
纯化方法	Purified
UniProt编号	P42336
UniProt汇总	FUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. SIZE: 1068 amino acids; 124284 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity). DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370]. SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain

产品使用声明
质量保证	Routinely evaluated by Western Blot on jurkat cell lysate. Western Blot Analysis: A 1:5,000 dilution of the lot detected endogenous PI3 Kinase, p110α in lysate from Jurkat cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.