merck millipore,默克密理博,04-220,Anti-Cyclin B1 (CT) Antibody, clone Y106, rabbit monoclonal

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merck millipore,默克密理博,04-220,Anti-Cyclin B1 (CT) Antibody, clone Y106, rabbit monoclonal
产品名称：Anti-Cyclin B1 (CT) Antibody, clone Y106, rabbit monoclonal
产品型号：04-220
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin B1 (C-term) Antibody, clone Y106, rabbit More>>

merck millipore,默克密理博,04-220,Anti-Cyclin B1 (CT) Antibody, clone Y106, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-220,Anti-Cyclin B1 (CT) Antibody, clone Y106, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB, FC, ICC, IHC	Rb	Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	04-220
品牌系列	Upstate
商名	Upstate
描述	Anti-Cyclin B1 (CT) Antibody, clone Y106, rabbit monoclonal
Alternate Names	proliferating cell nuclear antigen DNA polymerase delta auxiliary protein
背景信息	Cyclins are, as their name suggests, expressed at specific times during the cell cycle, and play a crucial role in the regulation of the cell cycle. Cyclins associate with cyclin-dependent kinases (cdks), and the association is absolutely required for cdk activity. At the conclusion of the relevant phase of the cell cycle, cyclins are rapidly ubiquitinated and degraded, thus returning cdks to their inactive state. Cyclin A associates with cdk2, and together with cyclin E is involved in the G1/S transition. Cyclin A/cdk2 remains active during S-phase. Cyclin B associates with cdk1/cdc2 to regulate the G2/M boundary. The D-cyclins bind cdk4 and cdk6, and result in Rb phosphorylation. The cyclin D1 gene was found in chronic B cell lymphocytic leukemia, and was designated Bcl-1 in a previously used nomenclature.

产品信息
格式	Culture Supernatant
控制	HeLa whole cell lysate.
演示	Unpurified rabbit monoclonal IgG in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cyclin B1 (C-term) Antibody, clone Y106, rabbit is an antibody against Cyclin B1 (C-term) for use in FC, IC, IH, IH(P), IP & WB.
主要应用	Western Blotting Flow Cytometry Immunocytochemistry Immunohistochemistry
应用说明	Immunohistochemistry: 1:100 dilution from a previous lot was used on immunohistochemistry. Immunocytochemistry: 1:50 dilution from a previous lot was used on immunocytochemistry. Immunoprecipitation: 1:100 dilution from a previous lot was used on immunoprecipitation. Flow Cytometry: 1:100 dilution from a previous lot was used on flow cytometry.

生物信息
免疫原品种	Synthetic peptide identical to the C-terminus of human Cyclin B1.
表位	C-terminus
克隆	Y106
宿主	Rabbit
特异性	This antibody recognizes Cyclin B1 at the C-Terminus.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human. Not expected to react with mouse and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_031966.2
Entrez基因汇总	The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq]
基因符号	MGC8367 Cyclin
非反应性品种	Rat Mouse
纯化方法	Unpurified
UniProt编号	P14635
UniProt汇总	Function: Essential for the control of the cell cycle at the G2/M (mitosis) transition. Ref.10 Ref.11 Subunit structure: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Ref.1 Ref.5 Ref.6 Subcellular location: Cytoplasm. Nucleus. Centrosome. Ref.8 Developmental stage: Accumulates steadily during G2 and is abruptly destroyed at mitosis. Ref.1 Post-translational modification: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Ref.7 Sequence similarities: Belongs to the cyclin family. Cyclin AB subfamily.

产品使用声明
质量保证	Routinely evaluated by Western Blot on HeLa whole cell lysate. Western Blot Analysis: A 1:5,000-1:10,000 dilution of this lot was used to detect Cyclin B1 on HeLa whole cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.