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merck millipore,默克密理博,04-1556,Anti-MDM4 Antibody, clone 7A8
产品名称：Anti-MDM4 Antibody, clone 7A8
产品型号：04-1556
Use Anti-MDM4 Antibody, clone 7A8 (mouse monoclonal antibody) validated in WB to detect MDM4 also known as Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 bind More>>
merck millipore,默克密理博,04-1556,Anti-MDM4 Antibody, clone 7A8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 04-1556 描述 Anti-MDM4 Antibody, clone 7A8 Alternate Names - p53-binding protein Mdm4
- Double minute 4 protein
- MDM4-related protein 1
- Mdm2-like p53-binding protein
- Mdm4 p53 binding protein homolog (mouse)
- Mdm4, transformed 3T3 cell double minute 4, p53 binding<br />protein
- Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
- Protein Mdmx
- double minute 4, human homolog of
- p53-binding protein
- mouse double minute 4 homolog
- mouse double minute 4, human homolog of
背景信息 MDM4 (sometimes referred to as MDMX) is a member of the MDM family proteins. It is known to inhibit p53 and p73 mediated cell cycle arrest and apoptosis. MDM4 is amplified or overexpressed in 10-20% of tumors and 65% of retinoblastomas. The full-length protein has significant homology to MDM2 and can also bind to and inhibit p53. In both MDM2 and MDM4, this interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Other similar domains include the C-terminal RING finger motif. Although these proteins share similar domains, MDM2 and MDM4 are non-redundant p53 inhibitors, since normal levels of either protein does not compensate for the loss of the other. Recent evidence suggests complementary activities for these proteins with MDM4 regulating p53 activity, and MDM2 playing a key role in p53 stability. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-MDM4 Antibody, clone 7A8 (mouse monoclonal antibody) validated in WB to detect MDM4 also known as Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse). 主要应用 - Western Blotting
生物信息 免疫原品种 His-tagged recombinant protein corresponding to mouse MDM4. 表位 Unknown 克隆 7A8 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes MDM4. 同种型 IgG2bκ 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with human and mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_032601
基因符号 - MRP1
- MDM4
- HDMX
- MDMX
纯化方法 Protein G purfied UniProt编号 - O35618
UniProt汇总 FUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The short isoform is a more potent inhibitor of p53 activity than the long isoform.
SUBUNIT STRUCTURE: Binds to p53, p73 and MDM2.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: In all tissues tested. The short isoform is expressed in a variety of transformed cell lines.
DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
SEQUENCE SIMILARITIES: Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM4 in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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