merck millipore,默克密理博,04-1104,Anti-C/EBP α (NT) Antibody, clone EP708Y, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-1104,Anti-C/EBP α (NT) Antibody, clone EP708Y, rabbit monoclonal
产品名称：Anti-C/EBP α (NT) Antibody, clone EP708Y, rabbit monoclonal
产品型号：04-1104
Anti-C/EBP α (N-term) Antibody, clone EP708Y is a Rabbit Monoclonal Antibody for detection of C/EBP α (N-term) has been validated in WB, ICC, IP & FC.

merck millipore,默克密理博,04-1104,Anti-C/EBP α (NT) Antibody, clone EP708Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1104,Anti-C/EBP α (NT) Antibody, clone EP708Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	FC, WB, ICC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1104
描述	Anti-C/EBP α (NT) Antibody, clone EP708Y, rabbit monoclonal
Alternate Names	CCAAT/enhancer binding protein alpha C/EBP alpha CCAAT/enhancer binding protein (C/EBP), alpha
背景信息	The transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPβ, also known as NFIL6) is a highly conserved B-Zip family member. C/EBPβ regulates multiple genes involved in hepatic function, immune function and adipogenesis, and its functions are generally distinct from those of other C/EBP family members (alpha, gamma, delta, epsilon). C/EBPβ is present in cells as three alternate translation products: two transcription activating forms known as LAP1 and LAP2 (~35 and 32 kDa in rodents, ~42 kDa and 40 kDa in humans) and a truncated inhibitory form known as LIP (~20 kDa) which lacks the N-terminal transcription activation domain. C/EBP β is phosphorylated at multiple sites which are determinants of some of its activities (e.g. activation of c-fos, adipogenesis). A recent report shows that it is acetylated at Lys215/216, and others report that it can be sumoylated. C/EBPα may play a role as a tumor suppressor and has been shown to modulate the expression of leptin.

产品信息
格式	Unpurified
控制	U937 + LPS cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Anti-C/EBP α (N-term) Antibody, clone EP708Y is a Rabbit Monoclonal Antibody for detection of C/EBP α (N-term) has been validated in WB, ICC, IP & FC.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry
应用说明	Immunocytochemistry Analysis: A 1:250-500 dilution from a representative lot detected C/EBP α in HeLa cells. Flow Cytometry: A 1:30 dilution of a representative lot was used in flow cytometry. Immunoprecipitation Analysis: A 1:50 dilution from a representative lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in the N-terminus of human C/EBP α.
表位	N-terminus
克隆	EP708Y
宿主	Rabbit
特异性	This antibody recognizes both the 42 kDa and the 30 kDa isoforms.
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Human and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_004355.2
Entrez基因汇总	The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
基因符号	CEBP C/EBP-alpha
纯化方法	Unpurified
UniProt编号	P49715
UniProt汇总	FUNCTION: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. SUBUNIT STRUCTURE: Interacts with PRDM16 By similarity. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X. SUBCELLULAR LOCATION: Nucleus. SEQUENCE SIMILARITIES:Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain.

产品使用声明
质量保证	Evaluated by Western Blot on U937 + LPS cell lysates. Western Blot Analysis: A 1:10,000-50,000 dilution of this antibody was used to detect C/EBP α in U937 + LPS cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.