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merck millipore,默克密理博,04-1555,Anti-Mdmx Antibody, clone 8C6
产品名称：Anti-Mdmx Antibody, clone 8C6
产品型号：04-1555
Anti-Mdmx Antibody, clone 8C6 is a Mouse Monoclonal Antibody for detection of Mdmx also known as MDM4-related protein 1, Mdm2-like p53-binding protein & has been validated in WB, IP, ICC, IHC, FC.
merck millipore,默克密理博,04-1555,Anti-Mdmx Antibody, clone 8C6
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ChIP, WB, ICC, IHC, FC M Purified Monoclonal Antibody 描述 产品目录编号 04-1555 描述 Anti-Mdmx Antibody, clone 8C6 Alternate Names - p53-binding protein 2 <br />p53-binding protein Mdm4
- Double minute 4 protein
- MDM4-related protein 1
- Mdm2-like p53-binding protein
- Mdm4 p53 binding protein homolog (mouse)
- Mdm4, transformed 3T3 cell double minute 4, p53 binding protein
- Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mouse)
- Protein Mdmx
- double minute 4, human homolog of
- p53-binding protein
- mouse double minute 4 homolog
- mouse double minute 4, human homolog of
背景信息 Mdmx is a Mdm2-related protein that like Mdm2 has been shown to bind to and inhibit p53, although not as well as Mdm2. This interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Mdmx has been shown to counteract Mdm2-dependent p53 degradation and to stabilize p53 in its inactive form. Although Mdmx has significant structural similarity to Mdm2 E3 ubiquitin ligase, most studies have not been able to ascribe E3 ligase function to Mdmx. 产品信息 格式 Purified 控制 - MCF-7 cell lysate
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-Mdmx Antibody, clone 8C6 is a Mouse Monoclonal Antibody for detection of Mdmx also known as MDM4-related protein 1, Mdm2-like p53-binding protein & has been validated in WB, IP, ICC, IHC, FC. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
- Flow Cytometry
应用说明 Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratoryfor this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)
Flow Cytometry Analysis: A previous lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)生物信息 免疫原品种 His-tagged recombinant protein corresponding to human Mdmx. 表位 Unknown 克隆 8C6 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Mdmx. 同种型 IgG2bκ 品种反应性 Human Species Reactivity Note Proven to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002384.2
Entrez基因汇总 The human MDM4 gene(encodes Mdmx protein), which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Two transcript variants, one protein-coding and the other likely not to be protein-coding, have been found for this gene. [provided by RefSeq] 基因符号 - MDM4
- HDMX
- MDMX
- MRP1
纯化方法 Protein G purfied UniProt编号 - O15151
UniProt汇总 FUNCTION: Inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions.
SUBUNIT STRUCTURE: Binds to p53, p73 and MDM2.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: In all tissues tested, with high levels in thymus.
DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73. Region II contains most of a central acidic region and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc mediates the heterooligomerization with MDM2.
SEQUENCE SIMILARITIES: Belongs to the MDM2/MDM4 family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 SWIB domain.
MASS SPECTROMETRY: Molecular mass is 54863.3 Da from positions 1 - 490. Determined by MALDI.产品使用声明 质量保证 Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Mdmx on 10 µg of MCF-7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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