merck millipore,默克密理博,16-146,Anti-IRS1 Antibody, agarose conjugate

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merck millipore,默克密理博,16-146,Anti-IRS1 Antibody, agarose conjugate
产品名称：Anti-IRS1 Antibody, agarose conjugate
产品型号：16-146
This Anti-IRS1 Antibody, agarose conjugate is validated for use in IP for the detection of IRS1.

merck millipore,默克密理博,16-146,Anti-IRS1 Antibody, agarose conjugate

产品介绍

merck millipore,默克密理博,16-146,Anti-IRS1 Antibody, agarose conjugate

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
A	IP	Rb	Gel Immobilized	Polyclonal Antibody

描述
产品目录编号	16-146
品牌系列	Upstate
商名	Upstate
描述	Anti-IRS1 Antibody, agarose conjugate
概述	Anti-IRS-1 (06-248) covalently coupled to protein A agarose using dimethylpimelimidate.
Alternate Names	insulin receptor substrate 1
背景信息	Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

产品信息
格式	Gel Immobilized
控制	3T3/A31 mouse fibroblasts cell lysate.
演示	Gel immobilized rabbit polyclonal IgG bound to beads; provided as a 50% gel slurry in PBS containing 0.05% azide. Liquid suspension.

应用
应用	This Anti-IRS1 Antibody, agarose conjugate is validated for use in IP for the detection of IRS1.
主要应用	Immunoprecipitation
应用说明	Affinity Purification: The antibody-agarose may be useful for affinity purification of proteins that bind IRS1. Suitable for immunoprecipitation and affinity purification. Elution Buffer (IRS1 Released) SDS-Page sample buffer (100%) 0.5M Glycine, pH 2.0 (0%) 0.1M TEA, pH 11.5 (0%)

生物信息
免疫原品种	Carboxy-terminal 14 amino acid peptide of rat liver IRS1 (C-YASINFQKQPEDRQ).
表位	C-terminal of IRS1
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes IRS1, Mr 160-185kDa.
同种型	IgG
品种反应性	All
Species Reactivity Note	All
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_005544.1
基因符号	IRS-1 HIRS-1
UniProt编号	P35568
UniProt汇总	FUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). SIZE: 1242 amino acids; 131591 Da SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7. PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity). DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].

产品使用声明
质量保证	Routinely evaluated by immunoprecipitating IRS-1 from a RIPA lysate of 3T3/A31 mouse fibroblasts. Immunoprecipitation: 10-25 μg (20-50 μL of 50% slurry) of this lot was used to immunoprecipitate IRS1 from 500 μg of a RIPA lysate of 3T3/A31 mouse fibroblasts. After boiling in SDS-PAGE sample buffer, a small amount of the non-covalently bound IgG light chain (28 kDa) and heavy chain (55 kDa) may be observed.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Routinely evaluated by immunoprecipitating IRS-1 from a RIPA lysate of 3T3/A31 mouse fibroblasts.

Immunoprecipitation: 10-25 μg (20-50 μL of 50% slurry) of this lot was used to immunoprecipitate IRS1 from 500 μg of a RIPA lysate of 3T3/A31 mouse fibroblasts. After boiling in SDS-PAGE sample buffer, a small amount of the non-covalently bound IgG light chain (28 kDa) and heavy chain (55 kDa) may be observed.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.