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merck millipore,默克密理博,05-1588,Anti-U1-70K Antibody, clone 9C4.1
产品名称：Anti-U1-70K Antibody, clone 9C4.1
产品型号：05-1588
Use Anti-U1-70K Antibody, clone 9C4.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect U1-70K also known as small nuclear ribonucleoprotein 70kDa (U1).
merck millipore,默克密理博,05-1588,Anti-U1-70K Antibody, clone 9C4.1
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1588 描述 Anti-U1-70K Antibody, clone 9C4.1 Alternate Names - U1 snRNP 70 kDa
- small nuclear ribonucleoprotein 70kDa (U1)
- U1 small nuclear ribonucleoprotein 70 kDa
- small nuclear ribonucleoprotein 70kDa (RNP antigen)
背景信息 U1 small nuclear ribonucleoprotein 70 kDa (U1-70K) is one of three specific proteins found within the U1 snRNP core, which includes at least seven core proteins found in all U snRNPs and three specific proteins; U1-70K, U1-A, and U1-C. U1-70K is involved in mediating pre-mRNA splicing through binding the loop I region of U1-snRNA with its RPM (RNA recognition motif) binding domain. In addition, its RNA binding domain, U1-70K also contains two argine-rich regions, accented with acidic or serine residues, that is thought to have trans-acting functions. Alternative splicing results in 4 known isoforms. Truncated forms of U1-70K are unable to bind RNA. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Use Anti-U1-70K Antibody, clone 9C4.1 (Mouse Monoclonal Antibody) validated in WB, ICC to detect U1-70K also known as small nuclear ribonucleoprotein 70kDa (U1). 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution of a representative lot of this antibody has been shown to detect U1-70K in NIH/3T3, A431, and HeLa cells. 生物信息 免疫原品种 Recombinant protein corresponding to C-terminal domain of human U1-70K. 表位 C-terminal domain 克隆 clone 9C4.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes U1-70K at the C-terminal domain. 同种型 IgG2aκ 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_003080.2
基因符号 - U1AP
- U1RNP
- U1-70K
- U170K
- RNPU1Z
- SNRP70
- U1AP1
- RPU1
- snRNP70
- SNRNP70
纯化方法 Protein G purfied UniProt编号 - P08621
UniProt汇总 FUNCTION: Mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. The truncated isoforms cannot bind U1-snRNA.
SUBUNIT STRUCTURE: Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SFRS2IP, and ZRANB2.
SUBCELLULAR LOCATION: Nucleus.
PTM: The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.
MISCELLANEOUS: Major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus.
SEQUENCE SIMILARITIES: Contains 1 RRM (RNA recognition motif) domain.
SEQUENCE CAUTION: The sequence CAA28352.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA29964.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA29966.1 differs from that shown. Reason: Erroneous initiation.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected U1-70K on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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