merck millipore,默克密理博,04-1043,Anti-Cytochrome C Antibody, clone EP1326Y, rabbit monoclonal

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merck millipore,默克密理博,04-1043,Anti-Cytochrome C Antibody, clone EP1326Y, rabbit monoclonal
产品名称：Anti-Cytochrome C Antibody, clone EP1326Y, rabbit monoclonal
产品型号：04-1043
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cytochrome C Antibody, clone EP1326Y, Rabbit de More>>

merck millipore,默克密理博,04-1043,Anti-Cytochrome C Antibody, clone EP1326Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1043,Anti-Cytochrome C Antibody, clone EP1326Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1043
描述	Anti-Cytochrome C Antibody, clone EP1326Y, rabbit monoclonal
Alternate Names	cytochrome c, somatic cytochrome c
背景信息	Cytochrome C is a 15 kDa protein in mitochondria which functions as an electron carrier in the cell respiratory chain by interacting with redox partners of complex III and complex IV. Cytochrome C is highly conserved during evolution. Recent evidence shows that Cytochrome C translocates from mitochondria to the cytosol in the cells undergoing apoptosis and participates in the central control or executioner phase of cell death cascade. Caspase-9 (Apaf-3) and Apaf-1 bind to each other in the presence of Cytochrome C and dATP, an event that leads to Caspase-9 activation. This activation, along with Cytochrome C, plays a major role in the activation of DEVD-specific caspases such as Caspase-3 and DNA fragmentation. Apoptosis inhibitors, Bcl-2 or Bcl-xL, prevent the efflux of Cytochrome C from the mitochondria and Bax, a pro-apoptotic member of the Bcl-2 protein family, can induce Cytochrome C release.

产品信息
格式	Unpurified
控制	HeLa, COS, and L929 cell lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Cytochrome C Antibody, clone EP1326Y, Rabbit detects level of Cytochrome C & has been published & validated for use in WB, IC, IH(P) & IP.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:100 - 250 dilution from a previous lot detected Cytochrome C in human kidney tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminus of human Cytochrome C.
表位	N-terminus
克隆	EP1326Y
宿主	Rabbit
特异性	This antibody recognizes Cytochrome C.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react in human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_061820.1
Entrez基因汇总	This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq].
基因符号	HCS CYC
纯化方法	Unpurified
UniProt编号	P99999
UniProt汇总	FUNCTION: Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. SUBCELLULAR LOCATION: Mitochondrion matrix. PTM: Binds 1 heme group per subunit. DISEASE: Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation. SEQUENCE SIMILARITY: Belongs to the cytochrome c family.

产品使用声明
质量保证	Evaluated by Western Blotting on HeLa, COS, and L929 cell lysates. Western Blotting Analysis: 1:500 dilution of this antibody was used to detect Cytochrome C in HeLa, COS, and L929 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.