merck millipore,默克密理博,04-1018,Anti-Progesterone Receptor Antibody, clone YR85, rabbit monoclonal

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merck millipore,默克密理博,04-1018,Anti-Progesterone Receptor Antibody, clone YR85, rabbit monoclonal
产品名称：Anti-Progesterone Receptor Antibody, clone YR85, rabbit monoclonal
产品型号：04-1018
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1018,Anti-Progesterone Receptor Antibody, clone YR85, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1018,Anti-Progesterone Receptor Antibody, clone YR85, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1018
描述	Anti-Progesterone Receptor Antibody, clone YR85, rabbit monoclonal
Alternate Names	progesterone receptor Nuclear receptor subfamily 3 group C member 3
背景信息	The progesterone receptor (PR) is a steroid receptor that binds progesterone. Estrogen must be present in order to induce the progesterone receptor. There are two isoforms of PR: PR-A and PR-B which share similar binding afffinities; however, they have opposite transcriptional activities.

产品信息
格式	Unpurified
控制	T47D cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:100-250 dilution from a previous lot detected Progesterone Receptor in human breast carcinoma tissue. Immunocytochemistry Analysis: A 1:50 dilution from a previous lot was used in IC. Immunoprecipitation Analysis: A 1:150 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near N-terminus of human Progesterone Receptor.
表位	N-terminus
克隆	YR85
宿主	Rabbit
特异性	The antibody does not cross-react with other NR3 family members.
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Human and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_000926.4
基因符号	PR NR3C3
纯化方法	Unpurified
UniProt编号	P06401
UniProt汇总	FUNCTION: The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. Isoform A is inactive in stimulating c-Src/MAPK signaling on hormone stimulation. SUBUNIT STRUCTURE: Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases. Isoform A: Nucleus. Cytoplasm. Note: Mainly nuclear. DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain. PTM: Phosphorylated on multiple serine sites. Several of these sites are hormone-dependent. Phosphorylation on Ser-294 occurs preferentially on isoform B, is highly hormone-dependent and modulates ubiquitination and sumoylation on Lys-388. Phosphorylation on Ser-102 and Ser-345 also requires induction by hormone. Basal phosphorylation on Ser-81, Ser-162, Ser-190 and Ser-400 is increased in response to progesterone and can be phosphorylated in vitro by the CDK2-A1 complex. Increased levels of phosphorylation on Ser-400 also in the presence of EGF, heregulin, IGF, PMA and FBS. Phosphorylation at this site by CDK2 is ligand-independent, and increases nuclear translocation and transcriptional activity. Phosphorylation at Ser-162 and Ser-294, but not at Ser-190, is impaired during the G2/M phase of the cell cycle. Phosphorylation on Ser-345 by ERK1/2 MAPK is required for interaction with SP1. Sumoylation is hormone-dependent and represses transcriptional activity. Sumoylation on all three sites is enhanced by PIAS3. Desumoylated by SENP1. Sumoylation on Lys-388, the main site of sumoylation, is repressed by ubiquitination on the same site, and modulated by phosphorylation at Ser-294. Ubiquitination is hormone-dependent and represses sumoylation on the same site. Promoted by MAPK-mediated phosphorylation on Ser-294. SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR3 subfamily. Contains 1 nuclear receptor DNA-binding domain.

产品使用声明
质量保证	Evaluated by Western Blot on T47D cell lysates. Western Blot Analysis: A 1:10,000 dilution of this antibody was used to detect Progesterone Receptor in T47D cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.