merck millipore,默克密理博,03-245,RIPAb+™ Hexim 2 - RIP Validated Antibody and Primer Set

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merck millipore,默克密理博,03-245,RIPAb+™ Hexim 2 - RIP Validated Antibody and Primer Set
产品名称：RIPAb+™ Hexim 2 - RIP Validated Antibody and Primer Set
产品型号：03-245
This RIPAb+ Hexim 2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-245,RIPAb+™ Hexim 2 - RIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,03-245,RIPAb+™ Hexim 2 - RIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
Chp, H	WB

描述
产品目录编号	03-245
商名	Upstate RIPAb+
描述	RIPAb+™ Hexim 2 - RIP Validated Antibody and Primer Set
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. Regulates transcription by acting as a general inhibitor of RNA polymerase II. When HEXIM1 is knocked down, HEXIM2 functionally compensates for its association with P-TEFb. HEXIM2 is up-regulated by HMBA (hexamethylene bisacetamide) at protein level.
Alternate Names	hexamthylene bis-acetamide inducible 2 Hexamethylene bis-acetamide-inducible protein 2 MAQ1 paralog hexamethylene-bis-acetamide-inducible transcript 2
背景信息	Regulates transcription by acting as a general inhibitor of RNA polymerase II. Similar to HEXIM1, HEXIM2 forms a complex with p-TEFb and 7SK that prevents p-TEFb from activating transcriptional elongation. When HEXIM1 is knocked down, HEXIM2 functionally compensates for its association with P-TEFb. HEXIM2 is up-regulated by HMBA (hexamethylene bisacetamide) at protein level.

产品信息
格式	Affinity Purified
控制	Includes negative control normal rabbit IgG antibody and control primers specific for the cDNA of human 7SK snRNA.
演示	Anti-Hexim 2 (Rabbit Polyclonal), . One vial containing 50 µg of affinity purified polyclonal in buffer containing 0.1 M Tris-glycine, 0.105 M NaCl, pH 7.4, and 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C. Normal Rabbit IgG, . One vial containing 125 μg of rabbit IgG in 125 μL of storage buffer containing 0.05% sodium azide. Store at -20°C. RIP Primers, 7SK snRNA. One vial containing 75 μL of 5 μM of each primer specific for the cDNA of 7SK snRNA. Store at -20°C. FOR: TCG GTC AAG GGT ATA CGA GTA GCT REV: CCC TAC GTT CTC CTA CAA ATG GA

应用
应用	This RIPAb+ Hexim 2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Western Blotting
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-Hexim 2 antibody (Cat. # CS207314). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: Hexim 2) and HeLa lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Hexim 2 antibody (Cat. # CS207314, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) Arrow indicates Hexim 2 at 42 kDa. (Figure 2). Western Blot Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-Hexim 2 (1:10,000) dilution. Proteins were visualized using a Donkey anti-Rabbit conjugated to HRP using a chemiluminescence detection system. Arrow indicates Hexim 2 (~42 kDa). (Figure 3).

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human Hexim 2 at and around the N-terminus.
表位	N-terminus
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	This antibody recognizes Hexim 2 at the N-terminus.
品种反应性	ChimpanzeeHuman
Species Reactivity Note	Demonstrated to react with human. Predicted to react with chimpanzee based on 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_653209
基因符号	L3 HEXIM2
纯化方法	Affinity Purfied
UniProt编号	Q96MH2
UniProt汇总	FUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. SUBUNIT STRUCTURE: Homo-oligomer and heterooligomer with HEXIM1; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in testis. HEXIM1 and HEXIM2 are differentially expressed. INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). DOMAIN: The coiled-coil domain mediates oligomerization. SEQUENCE SIMILARITIES: Belongs to the HEXIM family.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: Representative lot data. RIP Lysate prepared from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG (Cat. # PP64B), or 5 µg of Anti-Hexim 2 antibody (Cat. # CS207314) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Hexim 2-associated RNA was verified by qPCR using RIP Primers 7SK snRNA, (Cat. # CS204336) (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
Representative lot data.
RIP Lysate prepared from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG (Cat. # PP64B), or 5 µg of Anti-Hexim 2 antibody (Cat. # CS207314) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Hexim 2-associated RNA was verified by qPCR using RIP Primers 7SK snRNA, (Cat. # CS204336) (Figure 1).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.