merck millipore,默克密理博,03-232,Ubiquitin activating enzyme E1 (His-tag) human recombinant

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Merck Millipore 默克密理愽

主要应用	品种	Uni Prot编号
EA	Human	P22314

描述
产品目录编号	03-232
品牌系列	Upstate
商名	Upstate
描述	Ubiquitin activating enzyme E1 (His-tag) human recombinant
概述	Human recombinant Ubiquitin activating enzyme E1 (His-tag)
背景信息	Ubiquitin activating enzyme E1 is responsible for the first step in the formation of the ubiquitin-protein isopeptide bond. It is a critical component for the initiation of in vitro conjugation reactions. E1 activates ubiquitin by first adenylating, with ATP, the C-terminal glycine carboxyl group of ubiquitin and thenlinking this residue to the sulphydryl side chain moiety of a cysteine residue in E1, by forming a high energy thiol ester bond and liberating free AMP. There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.

产品信息
格式	Purified
演示	In liquid containing 50 mM Tris-HCl, pH 7.5, containing 1.0 mM DTT

应用
应用	Human recombinant Ubiquitin activating enzyme E1 (His-tag).
主要应用	Enzyme Assay

生物信息
宿主	E. coli
来源	Full length human ubiquitin-activating enzyme E1 containing a C-terminal His-tag
品种	Human
物理形式	Frozen solution
UniProt编号	P22314

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 12 months at -80°C from date of receipt. Avoid multiple freeze/thaw cycles.

包装信息
数量	50 µg

merck millipore,默克密理博,03-232,Ubiquitin activating enzyme E1 (His-tag) human recombinant

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