merck millipore,默克密理博,03-204,RIPAb+™ hnRNPA1 - RIP Validated Antibody and Primer Set

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merck millipore,默克密理博,03-204,RIPAb+™ hnRNPA1 - RIP Validated Antibody and Primer Set
产品名称：RIPAb+™ hnRNPA1 - RIP Validated Antibody and Primer Set
产品型号：03-204
This RIPAb+ hnRNPA1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-204,RIPAb+™ hnRNPA1 - RIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,03-204,RIPAb+™ hnRNPA1 - RIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
H	WB

描述
产品目录编号	03-204
商名	Upstate RIPAb+
描述	RIPAb+™ hnRNPA1 - RIP Validated Antibody and Primer Set
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. The hnRNP A1 protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may have a role in splice site selection.
Alternate Names	heterogeneous nuclear ribonucleoprotein A1 heterogeneous nuclear ribonucleoprotein A1B protein heterogeneous nuclear ribonucleoprotein B2 protein heterogeneous nuclear ribonucleoprotein core protein A1 hnRNP core protein A1 nuclear ribonucleoprotein particle A1 protein single-strand DNA-binding protein UP1 Helix-destabilizing protein Single-strand RNA-binding protein
背景信息	The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. While all hnRNPs are present in the nucleus, data suggests they shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by hnRNP A1 has two repeats of quasi-RRM domains that bind to RNAs. This abundant core protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. The hnRNP A1 protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may have a role in splice site selection.

产品信息
格式	Purified
控制	Includes negative control mouse IgG antibody and control primers specific for the mRNA of human E2F3.
演示	Anti-hnRNPA1 (Mouse Monoclonal), . One vial containing 50 μg of purified antibody in 75 μL of 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide and 30% glycerol. Store at -20°C . Normal Mouse IgG,. One vial containing 125 µg purified mouse IgG in 125 µL storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, E2F3 . One vial containing 75 μL of 5 μM of each primer specific for human E2F3 mRNA. Store at -20°C. FOR: TGG TAC CAT TGA GTT GCT GCT ATT REV: AGC TCA TGT GTT GCC CTT TAT ACA

应用
应用	This RIPAb+ hnRNPA1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Western Blotting
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal mouse IgG, (Cat. #CS200621), or 5 µg of Anti-hnRNPA1 antibody (Cat. # CS204408). Ten percent of the precipitated proteins (lane 1: mouse IgG, lane 3: hnRNPA1) and hela whole cell lysate (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-hnRNPA1 antibody (Cat. # CS204408, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) Arrow indicates hnRNPA1. (Figure 2). Arrow indicates hnRNPA1 (~38 kDa) (Figure 2). Western Blot Analysis: Representative lot data. K562 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-hnRNP A1, clone 4B10 (0.01 µg/mL dilution). Proteins were visualized using a Goat Anti-Mouse IgG conjugated to HRP using a chemiluminescence detection system. Arrow indicates hnRNP A1 (~38 kDa) (Figure 3).

生物信息
免疫原品种	Full length native protein partially purified human hnRNP A1.
表位	Unknown
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes hnRNP A1.
同种型	IgG2bκ
品种反应性	Human
Species Reactivity Note	Demonstrated to react with human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002127
Entrez基因汇总	This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variants have multiple alternative transcription initiation sites and multiple polyA sites. [provided by RefSeq].
基因符号	hnRNPA1 HNRNPA1 HNRPA1
纯化方法	Protein G purfied
UniProt编号	P09651
UniProt汇总	FUNCTION: Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRNPA1, HNRNPA2B1, HNRPA3, HNRNPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRNPR, HNRNPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with SEPT6. Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. PTM: Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine. SEQUENCE SIMILARITIES: Contains 2 RRM (RNA recognition motif) domains.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG ), or 5 µg Anti-hnRNPA1 antibody (Part. # CS204408) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of hnRNPA1-associated RNA was verified by qPCR using RIP Primers E2F3, (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG ), or 5 µg Anti-hnRNPA1 antibody (Part. # CS204408) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of hnRNPA1-associated RNA was verified by qPCR using RIP Primers E2F3, (Figure 1).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.