merck millipore,默克密理博,03-177,RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,03-177,RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set
产品名称：RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set
产品型号：03-177
This RIPAb+ Hexim 1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-177,RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,03-177,RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
Rhesus Macaque, H, Chp	WB

描述
产品目录编号	03-177
品牌系列	Upstate
商名	Upstate RIPAb+
描述	RIPAb+ Hexim 1 - RIP Validated Antibody and Primer Set
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. Hexim 1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim 1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.
Alternate Names	menage a quatre 1 Cardiac lineage protein 1 Estrogen down-regulated gene 1 protein Hexamethylene bis-acetamide-inducible protein 1 Menage a quatre protein 1 hexamethylene bis-acetamide inducible 1 hexamethylene bisacetamide-inducible protein hexamethylene-bis-acetamide-inducible transcript 1 hexamthylene bis-acetamide inducible 1
背景信息	Hexim1 is a transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. Hexim1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), Hexim1, Hexim2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. Hexim1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. Hexim1 is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.

产品信息
格式	Affinity Purified
控制	Includes negative control normal rabbit IgG antibody and control primers specific for the cDNA of human 7SK snRNA.
演示	Anti-Hexim 1 (Rabbit Polyclonal). One vial containing 50 µg of affinity purified polyclonal in 71 µL of buffer containing 0.1 M Tris-glycine, 150 mM NaCl, pH 7.4, and 0.05% sodium azide before the addition of 30% glycerol. Store at -20° C. Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C. RIP Primers, 7SK snRNA. One vial containing 75 μL of 5 μM of each primer specific for the cDNA of 7SK snRNA. Store at -20°C. FOR: TCG GTC AAG GGT ATA CGA GTA GCT REV: CCC TAC GTT CTC CTA CAA ATG GA

应用
应用	This RIPAb+ Hexim 1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Western Blotting
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 5 µg of a normal rabbit IgG or 5 µg of Anti-Hexim 1 antibody. Ten percent of the precipitated proteins (lane 3: rabbit IgG, lane 2: Hexim 1) and HeLa lysate (lane 1) were resolved by electrophoresis, transferred to PVDF and probed with anti-Hexim antibody (1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231) Arrow indicates Hexim 1 (~53 kDa) (Please see figures). Western Blot Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-Hexim 1 (1:10,000 dilution). Proteins were visualized using a Donkey anti-Rabbit conjugated to HRP using a chemiluminescence detection system. Arrow indicates Hexim 1 (~53 kDa) (Please see figures). Western Blot (SNAP ID®) Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-Hexim 1 (1:250 dilution). Proteins were visualized using a Donkey anti-Rabbit conjugated to HRP using a chemiluminescence detection system. Arrow indicates Hexim 1 (~53 kDa) (Please see figures).

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human Hexim 1 at and around the N-terminus.
表位	N-terminus
宿主	Rabbit
特异性	This antibody recognizes Hexim1 at the N-terminus.
品种反应性	Rhesus MacaqueHumanChimpanzee
Species Reactivity Note	Demonstrated to react with human. Predicted to react with chimpanzee and Rhesus monkey based on 100% sequence homology.
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_006451
Entrez基因汇总	Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.
基因符号	Hexim1 CLP-1 CLP1 EDG1 HIS1 HMBA-inducible MAQ1
纯化方法	Affinity Purfied
UniProt编号	O94992
UniProt汇总	FUNCTION: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. SUBUNIT STRUCTURE: Homo-oligomer and hetero-oligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: Binds alpha-importin and is mostly nuclear. TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. INDUCTION: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen. DOMAIN: The coiled-coil domain mediates oligomerization. MISCELLANEOUS: Inhibits Tat activity which is required for HIV-1 transcription. SEQUENCE SIMILARITIES: Belongs to the HEXIM family.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal rabbit IgG or 5 µg of Anti-Hexim 1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Hexim 1-associated RNA was verified by qPCR using RIP Primers 7SK snRNA (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal rabbit IgG or 5 µg of Anti-Hexim 1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Hexim 1-associated RNA was verified by qPCR using RIP Primers 7SK snRNA (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.