pall,颇尔,Blue Trisacryl M 填料,25896-045, 25896-010, 25896-028

For the Purification of a Wide
Variety of Enzymes and Proteins

• Strong bond of dye-to-sorbent prevents leakage of the dye in normal working conditions
• Excellent chemical stability is attributed to the synthetic nature of Trisacryl sorbent and the enhanced stability of the ligand coupling mechanism
• Excellent mechanical stability allows high flow rates with working pressures up to 3 bar (300 kPa, 45 psi)
• Basic matrix is Trisacryl GF2000, a macroporous non-ionic sorbent.Cibacron^♦ Blue F3GA dye is strongly bound to the matrix through a six-carbon spacer arm

• Albumin removal increases the resolution of both 1D and 2D electrophoresis
• Designed for the purification of a wide variety of enzymes and proteins such as kinases, interferons, and some coagulation factors
• The interaction mechanism between Cibacron Blue F3GA and proteins involves one or more of the following:stereospecific recognition of NAD analogs; electrostatic and hydrophobic interaction; and/or electron exchange

¹ Determined in PBS buffer using 5 mg/mL protein, column dimensions 16 mm I.D. x 6 cm bed height, flow rate 25 cm/hr

Analytical Separation of Human Plasma Proteins on Blue Trisacryl M Affinity Chromatography Sorbent  

Column:1.6 cm I.D. x 10 cm; Buffer:0.05 M Tris-HCI, pH 8.8; Elution performed by a continuous sodium chloride gradient from 0 to 3 M; Flow rate:100 cm/h; Separation time:180 min; Temperature:20 °C.

• AcroSep 亲合层析柱