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pall,颇尔,Biodyne 尼龙转印膜,60113, 60102, 60103, 60104, 60105, 60101, 60100, 60120, 60106, 60108, 60202, 60203, 60204, 60205, 60201, 60200, 60209, 60208, 60207, 60316, 60317, 60318, 60319, 60315, 60314, 604
产品名称:Biodyne 尼龙转印膜
产品型号:60113, 60102, 60103, 60104, 60105, 60101, 60100, 60120, 60106, 60108, 60202, 60203, 60204, 60205, 60201, 60200, 60209, 60208, 60207, 60316, 60317, 60318, 60319, 60315, 60314, 60402, 60403, 60404, 60405, 60401, 60400, 60406
pall,颇尔,Biodyne 尼龙转印膜,60113, 60102, 60103, 60104, 60105, 60101, 60100, 60120, 60106, 60108, 60202, 60203, 60204, 60205, 60201, 60200, 60209, 60208, 60207, 60316, 60317, 60318, 60319, 60315, 60314, 604
- 产品介绍

High Sensitivity and Low Background
for Enhanced Detection and Resolution- Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
- Membranes are intrinsically hydrophilic for easy wetting
- Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems
Four Chemistries Provide Versatile Adsorption Properties
Biodyne A Membrane
- (Amphoteric Nylon 6,6) Membrane zeta potential can be modulated by changes in pH.Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.
Biodyne B 膜
- (Positively-charged Nylon 6,6) Pore surfaces are populated by a high density of quaternary ammonium groups.Our highest sensitivity nylon membrane for nucleic acid applications.
Biodyne C 膜
- (Negatively-charged Nylon 6,6) Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.
Biodyne Plus 膜
- (Positively-charged Nylon 6,6 with an extremely high isoelectric point) With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.
过滤介质
- Biodyne A Membrane:两性尼龙 6,6膜
- Biodyne B and Plus Membranes:带正电荷的尼龙 6,6膜
- Biodyne C Membrane:Negativelycharged Nylon 6,6
孔径
- 0.2, 0.45, and 1.2 μm
Typical Thickness
膜 μm mils Biodyne A 5.5 - 7.0 139.7 - 177.8 Biodyne B 5.7 - 6.7 144.8 - 170.2 Biodyne Plus 5.7 - 6.7 144.8 - 170.2 Biodyne C 11.0 - 13.01 279.4 - 330.2 溶剂兼容性
- Resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2M NaOH, DMSO, and dimethylformamide.Not compatible with concentrated formic acid (> 50%), HCl (> 4M), oxidizing agents, and long exposures (days to weeks) to pH < 2.
1Dual layer measurements
Biodyne® B Membrane Withstands Multiple Cycles of Stripping and Reprobing

Lambda-HindIII fragments were separated in an agarose gel and transferred to Biodyne B membrane using the 颇尔 Improved Alkaline Transfer.The blot was stripped completely and reprobed four times without loss of signal intensity.Bands were detected using a chemiluminescent detection system.
1A - 4A:blot after (re)probing
1B - 4B:blot after stripping, prior to re(probing)Fluorescent Detection of DNA Using Biodyne Plus Membrane

ng total DNA
Dilutions of HindIII-digested I-DNA (1000 - 1 ng/lane) were separated in an agarose gel and transferred to Biodyne Plus membrane.Signal was generated using a fluorescein-labeled probe, antifluorescein-alkaline phosphatase conjugate, and precipitating substrate.The image was generated by scanning the blot with a FluorImager* system.*FluorImager is a registered trademark of Molecular Dynamics.
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