• pall,颇尔,Biodyne 尼龙转印膜,60113, 60102, 60103, 60104, 60105, 60101, 60100, 60120, 60106, 60108, 60202, 60203, 60204, 60205, 60201, 60200, 60209, 60208, 60207, 60316, 60317, 60318, 60319, 60315, 60314, 604
  • pall,颇尔,Biodyne 尼龙转印膜,60113, 60102, 60103, 60104, 60105, 60101, 60100, 60120, 60106, 60108, 60202, 60203, 60204, 60205, 60201, 60200, 60209, 60208, 60207, 60316, 60317, 60318, 60319, 60315, 60314, 604

    产品名称:Biodyne 尼龙转印膜
    产品型号:60113, 60102, 60103, 60104, 60105, 60101, 60100, 60120, 60106, 60108, 60202, 60203, 60204, 60205, 60201, 60200, 60209, 60208, 60207, 60316, 60317, 60318, 60319, 60315, 60314, 60402, 60403, 60404, 60405, 60401, 60400, 60406


    pall,颇尔,Biodyne 尼龙转印膜,60113, 60102, 60103, 60104, 60105, 60101, 60100, 60120, 60106, 60108, 60202, 60203, 60204, 60205, 60201, 60200, 60209, 60208, 60207, 60316, 60317, 60318, 60319, 60315, 60314, 604

  • 产品介绍
  • pall,颇尔,Biodyne 尼龙转印

    Biodyne 尼龙转印膜

    High Sensitivity and Low Background
    for Enhanced Detection and Resolution

    • Will not crack, shrink, or tear when subjected to multiple cycles of hybridization, stripping, and reprobing
    • Membranes are intrinsically hydrophilic for easy wetting
    • Offers superior performance with radioactive (Biodyne B membrane) and non-radioactive (Biodyne A membrane) detection systems
     
     

    Four Chemistries Provide Versatile Adsorption Properties

    Biodyne A Membrane

    • (Amphoteric Nylon 6,6) Membrane zeta potential can be modulated by changes in pH.Ideal for single probe or multiple rehybridizations, and applications where background is troublesome.

     

    Biodyne B 膜

    • (Positively-charged Nylon 6,6) Pore surfaces are populated by a high density of quaternary ammonium groups.Our highest sensitivity nylon membrane for nucleic acid applications.

     

    Biodyne C 膜

    • (Negatively-charged Nylon 6,6) Can be derivatized by coupling reactions through the carboxyl groups on the pore surfaces.

     

    Biodyne Plus 膜

    • (Positively-charged Nylon 6,6 with an extremely high isoelectric point) With certain nonradioactive detection systems, it is more sensitive than Biodyne A membrane while exhibiting lower background than Biodyne B membrane.
     
     

    过滤介质

    • Biodyne A Membrane:两性尼龙 6,6膜
    • Biodyne B and Plus Membranes:带正电荷的尼龙 6,6膜
    • Biodyne C Membrane:Negativelycharged Nylon 6,6

     

    孔径

    • 0.2, 0.45, and 1.2 μm

     

    Typical Thickness

     
    μm mils
    Biodyne A 5.5 - 7.0 139.7 - 177.8
    Biodyne B 5.7 - 6.7 144.8 - 170.2
    Biodyne Plus 5.7 - 6.7 144.8 - 170.2
    Biodyne C 11.0 - 13.01 279.4 - 330.2
     

     

    溶剂兼容性

    • Resistant to common solvents such as acetone, alcohol, chlorinated aliphatic hydrocarbons, formamide, 2M NaOH, DMSO, and dimethylformamide.Not compatible with concentrated formic acid (> 50%), HCl (> 4M), oxidizing agents, and long exposures (days to weeks) to pH < 2.

     

    1Dual layer measurements
     
     

    Biodyne® B Membrane Withstands Multiple Cycles of Stripping and Reprobing


    Biodyne B Membrane Withstands Stripping and Reprobing
    Lambda-HindIII fragments were separated in an agarose gel and transferred to Biodyne B membrane using the 颇尔 Improved Alkaline Transfer.The blot was stripped completely and reprobed four times without loss of signal intensity.Bands were detected using a chemiluminescent detection system. 

    1A - 4A:blot after (re)probing
    1B - 4B:blot after stripping, prior to re(probing)

     

    Fluorescent Detection of DNA Using Biodyne Plus Membrane


    Fluorescent Detection of DNA Using Biodyne Plus Membrane
    ng total DNA

     

    Dilutions of HindIII-digested I-DNA (1000 - 1 ng/lane) were separated in an agarose gel and transferred to Biodyne Plus membrane.Signal was generated using a fluorescein-labeled probe, antifluorescein-alkaline phosphatase conjugate, and precipitating substrate.The image was generated by scanning the blot with a FluorImager* system.*FluorImager is a registered trademark of Molecular Dynamics.
    Part Number 描述 Pkg Price Qty
    Biodyne A Membrane
    60113 0.2 µm, 30 cm x 3 m roll 1/pkg Call
    60102 0.45 µm, 82 mm discs 50/pkg Call
    60103 0.45 µm, 85 mm discs 50/pkg Call
    60104 0.45 µm, 132 mm discs 50/pkg Call
    60105 0.45 µm, 137 mm discs 50/pkg Call
    60101 0.45 µm, 7 x 8.5 cm sheets 10/pkg Call
    60100 0.45 µm, 20 x 20 cm sheets 10/pkg Call
    60120 0.45 µm, 20 cm x 3 m roll 1/pkg Call
    60106 0.45 µm, 30 cm x 3 m roll 1/pkg Call
    60108 1.2 µm, 30 cm x 3 m roll 1/pkg Call
    Biodyne B Membrane, 0.45 µm
    60202 82 mm discs 50/pkg Call
    60203 85 mm discs 50/pkg Call
    60204 132 mm discs 50/pkg Call
    60205 137 mm discs 50/pkg Call
    60201 7 x 8.5 cm sheets 10/pkg Call
    60200 20 x 20 cm sheets 10/pkg Call
    60209 20 cm x 1 m roll 1/pkg Call
    60208 20 cm x 3 m roll 1/pkg Call
    60207 30 cm x 3 m roll 1/pkg Call
    Biodyne C Membrane, 0.45 µm
    60316 82 mm discs 50/pkg Call
    60317 85 mm discs 50/pkg Call
    60318 132 mm discs 50/pkg Call
    60319 137 mm discs 50/pkg Call
    60315 7 x 8.5 cm sheets 10/pkg Call
    60314 20 x 20 cm sheets 10/pkg Call
    Biodyne Plus Membrane, 0.45 µm
    60402 82 mm discs 50/pkg Call
    60403 85 mm discs 50/pkg Call
    60404 132 mm discs 50/pkg Call
    60405 137 mm discs 50/pkg Call
    60401 7 x 8.5 cm sheets 10/pkg Call
    60400 20 x 20 cm sheets 10/pkg Call
    60406 30 cm x 3 m roll 1/pkg Call
     

     


    pall,颇尔,Biodyne 尼龙转印

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