pall,颇尔,HyperD F 离子交换填料（Q、S、DEAE 和 CM）,20067-C001, 20066-C001, 20062-C001, 20050-C001, 20066-031, 20066-023, 20066-015, 20062-030, 20062-022, 20062-014, 20067-039, 20067-021, 20067-013, 20050-035, 200

Preparative Sorbents for the Purification
of Biomolecules by Charge

• High binding capacity.“Gel-in-a-shell” design delivers outstanding dynamic capacity and exceptional dimensional stability for unsurpassed productivity.
• High flow rates.Proteins diffuse rapidly within the hydrogel, facilitating rapid uptake of product.This mechanism of mass transfer (known as “enhanced diffusion”) allows the sorbents to operate free of constraints.
• Abundant ion exchange sites in the hydrogel are highly accessible to protein molecules.
• Does not shrink or swell in response to changes in pH, ionic strength, or flow rate.
• Rigid, non-compressible sorbents are easy to pack.
• Easy cleaning with sodium hydroxide.
• Scalable from research and development to manufacturing.

• Direct capture of biomolecules from a variety of feedstocks
• Purification of polypeptides, IgG, and albumin
• Large-scale purifications
• Purification of monoclonal antibodies from ascites or cell culture
• 质粒纯化
• Process polishing steps

¹Sample:5 mg/mL BSA in 50 mM Tris-HCl buffer, pH 8.6
²Sample:5 mg/mL lysozyme in 50 mM sodium acetate, pH 4.5
³Sample:5 mg/mL Human IgG in 50 mM sodium acetate, 100 mM NaCl, pH 4.7

Dynamic Binding Capacity of Ceramic HyperD F Ion Exchange Resin Packed in 1 mL Chromatography Glass Columns at a Range of Flow Rates (Linear Velocities)

¹Dynamic binding capacity measured by breakthrough curve analysis at 10% of media saturation; a 1 mL volume column of ion exchange resin was packed and equilibrated with 25 mM Tris HCl pH 8.5 (anion ion exchange) or 10 mM MES-NaOH pH 5.5 (cation ion exchange) at the flow rates of 1, 5, or 10 mL/min.For anion ion exchange, 5 mg/mL BSA in the above buffer was then pumped onto the column until a break through in absorbance at 280 nm was seen.The flow was continued until a plateau in absorbance was achieved corresponding to 100% protein feed.Dynamic binding capacity was then calculated at 10% of the plateau value, correcting for any "dead volume" in the system and expressed as mg BSA/mL media volume.For cation ion exchange, 5 mg/mL lysozyme was used to test these resins in a similar manner to the anion ion exchange media.