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merck millipore,默克密理博,ST1029,PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4)
产品名称：PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4)
产品型号：ST1029
This PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4) is validated for use in ELISA, Immunoblotting for the detection of Fos (pSer³⁷⁴).
merck millipore,默克密理博,ST1029,PhosphoDetect™ Anti-Fos (pSer³⁷⁴) Mouse mAb (34E4)
- 产品介绍
重要规格表
宿主 M 描述 概述 Recognizes the ~55 kDa c-Fos protein phosphorylated at Ser374. Does not detect the unphosphorylated form. 产品目录编号 ST1029 品牌系列 Calbiochem®
应用数据 
Detection of human Fos, phosphorylated on Ser374, by immunoblotting. Samples: Serum starved HepG2 cells incubated with EGF for 0 min (lane 1), 5 min (lane 2), 15 min (lane 3), 30 min (lane 4), 1 h (lane 5), 2 h (lane 6), 4 h (lane 7), and 8 h (lane 8). Primary antibody: PhosphoDetect™ Anti-Fos (pSer374) Mouse mAb (34E4) (Cat. No. ST1029) (0.5 µg/ml). Detection: chemiluminescence.参考 参考 Chen, R-H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 10952. 产品信息 形式 Lyophilized 配方 100 µg antibody lyophilized from 2X PBS, PEG, sucrose and 200 µl lyophilized control lysate from EGF-treated HEPG2 cells. 正控制 HepG2 cells treated with EGF 防腐剂 ≤0.1% sodium azide (antibody only) 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁷⁴ phosphorylation site of human c-Fos 免疫原品种 Human 克隆 34E4 宿主 Mouse 同种型 IgG₁ 品种 human 储存和货运信息 货运代码 Shipped with Blue Ice or with Dry Ice
毒性 Irritant
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Reconstitute antibody with 1 ml water (15 minutes, room temperature). Following reconstitution, aliquot and freeze in liquid nitrogen. Reconstituted antibody can be stored at -80°C for up to 1 year. Aliquots may be stored at 4°C for up to 3 months and should be thawed at 37°C. Reconstitute control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconsitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
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