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品种反应性 主要应用 宿主 格式 抗体类型 M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3725 描述 Anti-PTF1A Antibody Alternate Names - pancreas transcription factor 1 subunit alpha
- Pancreas-specific transcription factor 1a
- bHLH transcription factor p48
- class II bHLH protein PTF1A
- exocrine pancreas-specific transcription factor p48
- p48 DNA-binding subunit of transcription factor PTF1
- pancreas specific transcription factor, 1a
- 1a
背景信息 The PTF1A (pancreas specific transcription factor, 1a) protein is a component of the pancreas transcription factor 1 complex (PTF1), and is known to play a critical role in the development of mammalian pancreatic stem cell lineages. More specifically, when expressed concurrently with the PDX-1 protein, PTF1A plays a role in determining whether cells allocated to the pancreatic progenitor pool continue on towards pancreatic organogenesis, or revert back to duodenal fates. The PTF1A protein is also thought to be involved in the maintenance of exocrine pancreas-specific gene expression, including elastase 1 and amylase. Further more, mutations in this gene have been shown to cause cerebellar agenesis, while the loss of PTF1A expression has been seen in ductal-type pancreatic cancers. 产品信息 格式 Affinity Purified 控制 - mouse brain lysates.
演示 Purified rabbit polyclonal in buffer containing PBS with 0.09% sodium azide. 应用 应用 Anti-PTF1A Antibody detects level of PTF1A & has been published & validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blotting:
Recommended working dilutions are 1:500 – 1:1000.生物信息 免疫原品种 Synthetic human PTF1A peptide, a.a. 301-317. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptided selected from human PTF1A. 品种反应性 Mouse Species Reactivity Note Reacts with mouse. Comparison of sequence homology suggests that this antibody may also react with human, canine, and bovine; however, reactivity with other species has not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_178161.2
Entrez基因汇总 This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq]
基因符号 - PTF1P48
- OTTHUMP00000019309
- PTF1-p48
纯化方法 Peptide affinity purified UniProt编号 - Q7RTS3
UniProt汇总 FUNCTION: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells (By similarity). Plays an important role in cerebellar development.
SUBUNIT: Component of the pancreas transcription factor 1 complex (PTF1) which is composed of TCF3/p75, TCF12/p64 and PTF1A/p48. TCF3 is responsible for the nuclear import of the p48/p64 complex. Interacts with TCF3 and RBPSUH/RBP-Jkappa (By similarity).
LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Note=In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex (By similarity).
SPECIFICITY: Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers.
DISEASE: Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis [MIM:609069].
MISCELLANEOUS: An excellent marker of acinar cell differentiation in the pancreas.
SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain.
产品使用声明 质量保证 Routinely evaluated by Western blot on mouse brain lysates. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles. 包装信息 数量 100 µL
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