merck millipore,默克密理博,SCR092,MilliTrace™ VM Constitutive GFP Reporter Human Neural Stem Cell Kit

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merck millipore,默克密理博,SCR092,MilliTrace™ VM Constitutive GFP Reporter Human Neural Stem Cell Kit
产品名称：MilliTrace™ VM Constitutive GFP Reporter Human Neural Stem Cell Kit
产品型号：SCR092
MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cell Kit.

merck millipore,默克密理博,SCR092,MilliTrace™ VM Constitutive GFP Reporter Human Neural Stem Cell Kit

产品介绍

merck millipore,默克密理博,SCR092,MilliTrace™ VM Constitutive GFP Reporter Human Neural Stem Cell Kit

重要规格表

主要应用
Cult

描述
产品目录编号	SCR092
品牌系列	Chemicon®
商名	Chemicon MilliTrace
描述	MilliTrace™ VM Constitutive GFP Reporter Human Neural Stem Cell Kit
概述	MilliTrace™ VM Constitutive GFP Reporter Human Neural Stem Cell Kit (Catalog No. SCR092) provides ready-to use ReNcell VM human neural stem cells that are constitutively labeled with the humanized mulleri green fluorescent protein (hmGFP) along with expansion medium to help maintain expression of the transgene. The parental cell line, ReNcell VM, is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. The ReNcell VM line was derived from the ventral mesencephalon region of a 10-week human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. ReNcell VM cells can be differentiated in vitro to human dopaminergic neurons. Neurons differentiated from ReNcell VM cells have been shown to be electrophysiologically active. MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cells were generated by electroporating the ReNcell VM line with a proprietary bicistronic plasmid construct containing hmGFP under the control of a constitutive chicken actin promoter. FACS analyses of stable transfectants indicate that greater than 95% of the cells express GFP at high levels even after 10 passages. MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cells display the immunochemical staining properties of neural stem cells (NSCs); they are positive for Nestin and Sox-2 expression. The cells are multipotent and are able to differentiate into neurons and astrocytes. Cells have been confirmed to be mycoplasma-free and demonstrate an apparently normal karyotype (46, XY) as assessed by standard G-banding analysis performed on 20 metaphase cells.

产品信息
部件	SCC092, 1 x 10⁶ viable MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cells: SCM043, MilliTrace ReNcell Neural Stem Cell Maintenance Media Kit

应用
应用	MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cell Kit.
主要应用	Stem Cell Culture

生物信息
品种	Human
细胞系类型	Neural Stem Cells

产品使用声明
质量保证	MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cells have been validated for high level constitutive expression of GFP, for high level expression of Nestin and Sox-2 in the NSC compartment and for their self-renewal and multi-lineage differentiation capacities (please refer to product manual figures for representative data). Cells display normal karyotype as assessed by G-banding of 20 metaphase cells and tested negative for mycoplasma.
使用声明	This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled. For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed.

产品使用声明

质量保证

MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cells have been validated for high level constitutive expression of GFP, for high level expression of Nestin and Sox-2 in the NSC compartment and for their self-renewal and multi-lineage differentiation capacities (please refer to product manual figures for representative data). Cells display normal karyotype as assessed by G-banding of 20 metaphase cells and tested negative for mycoplasma.

使用声明

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.
For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed.

储存和货运信息
存储条件	MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cells (Catalog No. SCC092) should be stored in liquid nitrogen. ReNcell Neural Stem Cell Maintenance Medium (Catalog No. SCM005) should be stored at -20oC until expiration date on the label. Upon thawing the basal medium should be stored at 2-8°C and given a 1-month expiration dating. 5 mg/mL Puromycin Solution, 100 L (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.

储存和货运信息

存储条件

MilliTrace VM Constitutive GFP Reporter Human Neural Stem Cells (Catalog No. SCC092) should be stored in liquid nitrogen.
ReNcell Neural Stem Cell Maintenance Medium (Catalog No. SCM005) should be stored at -20oC until expiration date on the label. Upon thawing the basal medium should be stored at 2-8°C and given a 1-month expiration dating.
5 mg/mL Puromycin Solution, 100 L (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.