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merck millipore,默克密理博,S8001M,CpGenome™ Human Methylated DNA Standard Set
产品名称：CpGenome™ Human Methylated DNA Standard Set
产品型号：S8001M
It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
merck millipore,默克密理博,S8001M,CpGenome™ Human Methylated DNA Standard Set
- 产品介绍
描述 产品目录编号 S8001M 品牌系列 Upstate
商名 - Upstate
- CpGenome
描述 CpGenome™ Human Methylated DNA Standard Set 概述 The CpGenome™ Human Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. This Standard has been methylated enzymatically at all CpG dinucleotides by M. SssI methyltransferase. It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome™ Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ® amplification kits.
Materials Provided:
One vial containing 5 µg (20 µL) of CpGenome™ Human Methylated DNA Standard at a concentration of 250 ng/µL.
Validation:
Methylation-specific PCR (MSP) was performed on the CpGenome™ Human Methylated DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the M primers generated product for the methylated DNA standard.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.产品信息 演示 Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 应用 应用 It is intended for use as a positive control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit. 应用说明 For MSP primer design, please use the MethPrime software package. 生物信息 品种反应性 Human 修改 - Methylation
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Recommended Storage: Stable at -20°C for up to 6 months from date of receipt. 包装信息 数量 5 µg
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