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品种反应性 主要应用 宿主 格式 抗体类型 R WB, IHC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5378-200UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-HCN2 Antibody Alternate Names - HAC-1
- Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 2
- BCNG2
产品信息 格式 Affinity Purified 控制 - CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). 应用 应用 Detect HCN2 using this Anti-HCN2 Antibody validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western blot: 1:200-1:1,000 using ECL on rat brain membranes.
Immunohistochemistry on rat brain membrane sections.
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Purified peptide from human HCN2 (amino acids 147-161) (Accession CAB42602). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes HCN2 protein. The epitope is specific for HCN2 and is not present in any other known protein. 品种反应性 Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001194.3
基因符号 - BCNG2
- HCN2
- BCNG-2
- HAC-1
UniProt编号 - Q9UL51
UniProt汇总 FUNCTION: SwissProt: Q9UL51 # Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity).
SIZE: 889 amino acids; 96950 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed throughout the brain. Detected at low levels in heart.
DOMAIN:SwissProt: Q9UL51 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
MISCELLANEOUS: Inhibited by extracellular cesium ions.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 200 µL
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