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  • merck millipore,默克密理博,S7820,CpGenome Universal DNA Modification Kit

  • merck millipore,默克密理博,S7820,CpGenome Universal DNA Modification Kit

    产品名称：CpGenome Universal DNA Modification Kit
    产品型号：S7820
    Kit containing all reagents required to perform DNA bisulfite modifications for DNA methylation experiments and methylation-specific PCR (MSP).

    merck millipore,默克密理博,S7820,CpGenome Universal DNA Modification Kit

  • 产品介绍
  • merck millipore,默克密理博,S7820,CpGenome Universal DNA Modification Kit

    描述
    产品目录编号 S7820
    品牌系列 Chemicon®
    商名
    • Chemicon
    • CpGenome
    描述 CpGenome Universal DNA Modification Kit
    概述 The CpGenome™ DNA Modification Kit is used to create methylation-specific sequence alterations in any DNA sample. Using chemical treatment, the kit converts all unmethylated cytosine residues to uracil. Methylated cytosine residues are unaltered in this process. Therefore, the chemically-treated DNA sequence will differ only if it was originally unmethylated in vivo. After sequence conversion, methylation specific PCR is employed to detect these differences using primers specific for any gene of interest (or the CpG™ Wiz Amplification Kit Primers).
    Alternate Names
    • Bisulfite Modification Kit
    背景信息 Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppresser genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors.
    Several methods have been developed to determine the methylation status of cytosine. These include digestion with methylation sensitive restriction enzymes as in restriction landmark genomic scanning, oligonucleotide arrays, genomic DNA sequencing and methylation specific PCR (MSP). Some techniques are more useful for discovery while others are better used for monitoring of known methylated cytosines. Genomic DNA sequencing, although time consuming and labor intensive, offers a more universal detection method. MSP is now an established technology for the monitoring of abnormal gene methylation in selected gene sequences. Utilizing small amounts of DNA, this procedure offers sensitive and specific detection of 5-methylcytosine in promoters. It is being exploited to define tumor suppresser gene function, and to provide a new strategy for early tumor detection.
    The initial step of both genomic sequencing and MSP is to perform a bisulfite modification of the DNA sample. MSP then involves PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.
    应用
    应用 Kit containing all reagents required to perform DNA bisulfite modifications for DNA methylation experiments and methylation-specific PCR (MSP).
    主要应用
    • Software Needed
    应用说明 For MSP primer design, please use the MethPrime software package.
    生物信息
    品种反应性 Human
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    包装信息
    数量 100 units
    包装 100 samples

    merck millipore,默克密理博,S7820,CpGenome Universal DNA Modification Kit

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