- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
检测方法 Colorimetric 描述 概述 Non-isotopic kit for the detection of apoptosis at the individual cell level. Apoptotic morphology is easily detected. This kit allows the recognition of apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, or in cell preparations fixed on slide by Fragment End Labeling (FragEL™) of DNA. Klenow binds to exposed ends of DNA fragments generated in response to apoptotic signals and catalyzes the template-dependent addition of biotin-labeled and unlabeled deoxynucleotides. Biotinylated nucleotides are detected using streptavidin-horseradish peroxidase (HRP) conjugate. Diaminobenzidine reacts with the labeled sample to generate an insoluble colored product at the site of DNA fragmentation. Counterstaining with methyl green aids in the morphological evaluation and characterization of normal and apoptotic cells. 产品目录编号 QIA21 品牌系列 Calbiochem®
同义词 TUNEL Assay 参考 参考 Xiao, D. and Bullock, R. 1996. J. Neurosurgery 85, 655.
Gu, Y., et al. 1994. Endocrinology 135, 1272.
Gavrieli, Y., et al. 1992. J. Cell Biol. 119, 493.
Fawthrop, D. J., et al. 1991. Arch. Toxicol. 65, 437.
Martin, S.J., et al. 1990. J. Immunology 145, 1859.
Wyllie, A. H. 1980. Nature 284, 555.
Kerr, J. F. R., et al. 1972. Br. J. Cancer 26, 239.产品信息 检测方法 Colorimetric 形式 50 Tests 格式 Microscopy 试剂盒包含 Counterstain, Proteinase K, Equilibration and Labeling Buffers, Klenow Enzyme (Cat. No. PF059), Stop buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H₂O₂/Urea Tablets, Methyl Green Counterstain, and a user protocol. 应用 主要应用 Frozen Sections
Paraffin Sections
生物信息 化验时间 4 h 样品类型 Frozen or paraffin sections or fixed-cell preparations 品种 a broad range of species 产品使用声明 预定用途 The Calbiochem® Klenow-FragEL™ DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, and in cell preparations fixed on slides. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 -20°C
存储条件 Upon arrival store the entire contents of the kit at -20°C in a non-frostfree freezer. Following initial thaw store the Stop Buffer and Methyl Green Counterstain at room temperature and the Blocking Buffer at 4°C. Refreezing of these components, however, does not appear to affect their performance. 避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Counterstain, Proteinase K, Equilibration and Labeling Buffers, Klenow Enzyme (Cat. No. PF059), Stop buffer, Blocking Buffer, 50X Conjugate, DAB, Positive and Negative Control Slides, H₂O₂/Urea Tablets, Methyl Green Counterstain, and a user protocol.
上一件merck millipore产品：merck millipore,默克密理博,SF-1636,IL1A; Human, Cyanine 3 RNA Detection Probe,SmartFlare
下一件merck millipore产品：merck millipore,默克密理博,MAB8430,Anti-Enterovirus Antibody, 70/71 Blend, clone 74-5G and 422-8D-4C-4D
相关产品
merck millipore,默克密理博,AB15588,Anti-DCAMKL1 splice variant Antibody
merck millipore,默克密理博,AB119,Anti-Dopa Decarboxylase Antibody
merck millipore,默克密理博,AB2200,Anti-PRG4 Antibody
merck millipore,默克密理博,71333,pIEx™-6 DNA - Novagen
merck millipore,默克密理博,SF-1636,IL1A; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,MAB8430,Anti-Enterovirus Antibody, 70/71 Blend, clone 74-5G and 422-8D-4C-4D
merck millipore,默克密理博,05-1250,Anti-Trimethyl Histone H3(Lys9) Antibody, clone CMA308
merck millipore,默克密理博,14-404,ZAP-70 Protein, active, 20 µg
merck millipore,默克密理博,CC1019,Human Integrin αVβ3, Triton X-100 Formulation
merck millipore,默克密理博,MABC686,Anti-IRF-5 Antibody, clone EPR6094, rabbit monoclonal