• merck millipore,默克密理博,PS1008,PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb
  • merck millipore,默克密理博,PS1008,PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

    产品名称:PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb
    产品型号:PS1008
    This PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr⁸⁶¹).

    merck millipore,默克密理博,PS1008,PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

  • 产品介绍
  • merck millipore,默克密理博,PS1008,PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

    重要规格表

    宿主
    Rb
    描述
    概述 Recognizes the ~125 kDa FAK protein phosphorylated at Tyr861 in primary chick embryo fibroblasts transfected with human FAK.
    产品目录编号 PS1008
    品牌系列 Calbiochem®
    同义词 Anti-FADK1, Phospho-Specific (Tyr⁸⁶¹), Anti-pp125FAK, Phospho-Specific (Tyr⁸⁶¹), Anti-Protein Tyrosine Kinase 2, Phospho-Specific (Tyr⁸⁶¹), Anti-PTK2, Phospho-Specific (Tyr⁸⁶¹), FAK 1
    应用数据
    Detection of FAK, phosphorylated at Tyr861, by immunoblotting. Samples: Whole cell lysates from chicken embryo fibroblasts (CEF) expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr861) Rabbit pAb (Cat. No. PS1008) (1:1000) pre-incubated with phosphopeptide immunogen (lane 1), a generic phosphotyrosine-containing peptide (lane 2), non-phosphorylated peptide corresponding to the phosphopeptide immunogen (lane 3), or without peptide (lane 4). Detection: chemiluminescence.
    参考
    参考 Eliceiri, B.P., et al. 2002. J. Cell. Biol. 157, 149.
    Hauck, C.R., et al. 2002. J. Biol. Chem. 277, 12487.
    Lacalle, R.A., et al. 2002. J. Cell. Biol. 157, 277.
    Lim, Y., et al. 2002. J. Biol. Chem. 277, 12735.
    Melendez, J., et al. 2002. J. Biol. Chem. 227, 45203.
    Rigacci, S., et al. 2002. J. Biol. Chem. 277, 41631.
    Datta, A., et al. 2001. Mol. Cell. Biol. 21, 7295.
    Nakamura, K., et al. 2001. Oncogene 20, 2626.
    Rocic, P., et al. 2001. J. Biol. Chem. 276, 21902.
    Slack, J.K., et al. 2001. Oncogene 20, 1152.
    Sieg, D.J., et al. 2000. Nature Cell. Biol. 2, 249.
    Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
    Wennerberg, K., et al 2000. Mol. Cell. Biol. 20, 5758.
    产品信息
    形式 Liquid
    配方 In Dulbecco's PBS (without Mg2+ and Ca2+), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
    正控制 Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
    防腐剂 ≤0.1 sodium azide
    应用
    主要应用 Immunoblotting (Western Blotting)
    Immunohistochemistry
    生物信息
    免疫原 a synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
    免疫原品种 Human
    宿主 Rabbit
    同种型 IgG
    品种 chicken, human, mouse
    储存和货运信息
    货运代码 Shipped with Blue Ice or with Dry Ice
    毒性 Irritant
    储存 -20°C
    避免冻结 / 解冻 Avoid freeze/thaw
    无需冷冻 No
    特殊指南 Following initial thaw, aliquot and freeze (-20°C).

    merck millipore,默克密理博,PS1008,PhosphoDetect™ Anti-FAK (pTyr⁸⁶¹) Rabbit pAb

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