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merck millipore,默克密理博,PK1008,PhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb
产品名称：PhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb
产品型号：PK1008
This PhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb is validated for use in FC, Immunoblotting, IP, Paraffin Sections for the detection of PDGFRβ(pTyr⁷⁵¹).
merck millipore,默克密理博,PK1008,PhosphoDetect™ Anti-PDGFRβ(pTyr⁷⁵¹) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~190 kDa PDGF receptor-β protein phosphorylated at Tyr751 in PDGF-stimulated L6, HepG2, and NIH3T3 cells. 产品目录编号 PK1008 品牌系列 Calbiochem®
同义词 Anti-Platelet Derived Growth Factor Receptor-β, Phospho-Specific (Tyr⁷⁵¹) 应用数据 
Detection of mouse and human PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Samples: Whole cell lysate from L6, HepG2, and NIH3T3 cells left untreated (-) or treated with PDGF (40 ng/ml for 5 min; +). Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1007) (1:1000). Detection: chemiluminescence.
Detection of human PDGFR-β, phosphorylated on Tyr751, by staining paraffin sections. Sample: Breast carcinoma tissue. Primary antibody: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:50). Detection: DAB with hematoxylin counterstain.
Detection of mouse PDGFR-β, phosphorylated on Tyr751, by immunoblotting. Sample: NIH3T3 cells left untreated (blue) or treated with PDGF (green). Primary antibodies: PhosphoDetect™ Anti-PDGFR-β (pTyr751) Rabbit pAb (Cat. No. PK1008) (1:200) and irrelevant control antibody (red). Detection: fluorescence.参考 参考 Betsholtz, C., et al. 2001. Bioessays 23, 494.
Ostman, A. and Heldin, C.H. 2001. Adv. Cancer Res. 80, 1.
Ramalingam, K., et al. 1995. Bioorg. Med. Chem. 3, 1263.
Panyotou, G. et al. 1992. EMBO J. 11, 4261.
Coughlin, S.R., et al. 1988. Prog. Clin. Biol. Res. 266, 39.
Deuel, T.F., et al. 1988. Biofactors 1, 213.产品信息 形式 Liquid 配方 In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. 正控制 Breast carcinoma and PDGF-treated L6, HepG2, and NIH3T3 cells 防腐剂 None 应用 主要应用 Flow Cytometry
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁷⁵¹ phosphorylation site of human PDGF receptor-β, coupled to KLH 免疫原品种 Human 宿主 Rabbit 同种型 IgG1 品种 human, mouse, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
无需冷冻 No
特殊指南 Do not aliquot.
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