merck millipore,默克密理博,OP43L,Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

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merck millipore,默克密理博,OP43L,Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)
产品名称：Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)
产品型号：OP43L
This Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1) is validated for use in Frozen sections, Gel Shift, Immunoblotting, ICC, IP, Paraffin sections for the detection of p53 (Ab-6) (Pantropic).

merck millipore,默克密理博,OP43L,Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

产品介绍

merck millipore,默克密理博,OP43L,Anti-p53 (Ab-6) (Pantropic) Mouse mAb (DO-1)

重要规格表

宿主
M

描述
概述	Recognizes the ~53 kDa wild-type and mutant p53 protein in A431 cells and breast carcinoma tissue.
产品目录编号	OP43L
品牌系列	Calbiochem®

参考
参考	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Legros, Y., et al. 1994. Oncogene 9, 2071. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

参考

El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Legros, Y., et al. 1994. Oncogene 9, 2071.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237.
Kastan, M.B., et al. 1991 Cancer Res. 51, 6304.

产品信息
形式	Lyophilized
配方	Lyophilized from a volatile buffer, 100 µg BSA.
负控制	SK-OV-3 cells or normal skin tissue
正控制	A431 cells or breast carcinoma tissue
防腐剂	None

应用
应用参考	Immunoprecipitation, Immunocytochemistry Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Paraffin Sections, Frozen Sections, Original Clone Vojtesek, B., et al. 1992. J. Immunol. Meth. 151, 237. Immunoblotting Loganzo, F., et al. 1994. Mol. Cell. Different. 2, 23. Epitope Legros, Y., et al. 1994. Oncogene 9, 2071.
主要应用	Frozen Sections Gel Shift Immunoblotting (Western Blotting) Immunocytochemistry Immunoprecipitation Paraffin Sections

生物信息
免疫原	wild type, recombinant, human p53
免疫原品种	Human
表位	Within amino acids 21-25 of human p53
克隆	DO-1
宿主	Mouse
同种型	IgG_2a
品种	feline, human, not mouse, not rat

储存和货运信息
货运代码	Ambient Temperature Only
毒性	Standard Handling
储存	+2°C to +8°C
无需冷冻	No
特殊指南	We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 μg/ml. Reconstituted product will be more stable if 0.1% sodium azide is added (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two hours. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.