merck millipore,默克密理博,OP29L,Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)

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Merck Millipore 默克密理愽

宿主
M

描述
概述	Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions.
产品目录编号	OP29L
品牌系列	Calbiochem®

参考
参考	El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169. Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855. Barak, Y., et al. 1993. EMBO J. 12, 461. Kastan, M.B., et al. 1992. Cell 71, 587. Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491. Lane, D.P. 1992. Nature 358, 15. Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

产品信息
形式	Lyophilized
配方	Lyophilized from a volatile buffer, 100 µg BSA.
负控制	SK-OV-3 cells
正控制	A431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma
防腐剂	None

应用
应用参考	Paraffin Sections Pezzella, F., et al. 1994. J. Clin. Pathol. 47, 592. Original Clone Gannon, J.V., et al. 1990. EMBO J. 9, 1595. Epitope Identification Stephen, C.W. and Lane, D.P., 1992. J. Mol. Biol. 225, 1. Immunofluorescence Bernardi, R., et al. 2004. Nat. Cell Biol. 6, 665.
主要应用	Flow Cytometry Frozen Sections Gel Shift Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Paraffin Sections

生物信息
免疫原	a recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
免疫原品种	Human
表位	within amino acids 213-217
克隆	PAb240
宿主	Mouse
同种型	IgG₁
品种	not Xenopus, bovine, chicken, hamster, human, mouse, rat

储存和货运信息
货运代码	Ambient Temperature Only
毒性	Standard Handling
储存	+2°C to +8°C
无需冷冻	No
特殊指南	Reconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide

merck millipore,默克密理博,OP29L,Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)

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