merck millipore,默克密理博,OP15L,Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

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merck millipore,默克密理博,OP15L,Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)
产品名称：Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)
产品型号：OP15L
This Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-ErbB2/c-Neu (Ab-3).

merck millipore,默克密理博,OP15L,Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

产品介绍

merck millipore,默克密理博,OP15L,Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)

重要规格表

宿主
M

描述
概述	Recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells.
产品目录编号	OP15L
品牌系列	Calbiochem®
同义词	Anti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-Human Epidermal Growth Factor
应用数据	Detection of human c-ErbB2/c-Neu by immunoblotting. Sample: Whole cell lysate from HEK293 cells. Primary antibody: Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5) (Cat. No. OP15L) (1 µg/ml). Detection: chemiluminescence.

参考
参考	Mamcs, J.R., et al. 1994. Ann. Surg. 219, 332. Tetu, B., et al. 1994. Cancer 73, 2359. DiFiore, P.P., et al. 1987. Science 237, 178. Slamon, D.J., et al. 1987. Science 235, 177. Varley, J.M., et al. 1987. Oncogene 1, 423. Bargmann, C.I., et al. 1986. Nature 319, 226. Yamamoto, T., et al. 1986. Nature 319, 230. Blick, M., et al. 1984. Blood 64, 1234. Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

参考

Mamcs, J.R., et al. 1994. Ann. Surg. 219, 332.
Tetu, B., et al. 1994. Cancer 73, 2359.
DiFiore, P.P., et al. 1987. Science 237, 178.
Slamon, D.J., et al. 1987. Science 235, 177.
Varley, J.M., et al. 1987. Oncogene 1, 423.
Bargmann, C.I., et al. 1986. Nature 319, 226.
Yamamoto, T., et al. 1986. Nature 319, 230.
Blick, M., et al. 1984. Blood 64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

产品信息
形式	Lyophilized
配方	Lyophilized from 20 mM ammonium bicarbonate Buffer, 100 µg FAF-BSA.
负控制	HepG2 cells or normal skin
正控制	SK-BR-3 cells or breast carcinoma tissue
防腐剂	None

应用
应用参考	Paraffin Sections Meissner, K., et al. 1990. Am. J. Pathol. 137, 1305. Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Original Clone Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Immunohistochemistry, Immunoblotting Zhau, H.E., et al. 1990. Mol. Carcinogen. 3, 254. Immunoprecipitation Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239.
主要应用	Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Paraffin Sections

应用

应用参考

Paraffin Sections Meissner, K., et al. 1990. Am. J. Pathol. 137, 1305. Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Original Clone Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239. Immunohistochemistry, Immunoblotting Zhau, H.E., et al. 1990. Mol. Carcinogen. 3, 254. Immunoprecipitation Van De Vijver, J.J., et al. 1988. New Engl. J. Med. 319, 1239.

主要应用

Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Paraffin Sections

生物信息
免疫原	a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
免疫原品种	Human
克隆	3B5
宿主	Mouse
同种型	IgG₁
品种	human, mouse

储存和货运信息
货运代码	Ambient Temperature Only
毒性	Standard Handling
储存	+2°C to +8°C
无需冷冻	No
特殊指南	Reconstitue the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long term storage of reconstituted product; repetitive freezing and thawing should be avoided.