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merck millipore,默克密理博,MIT1000,Mitochondria/Cytosol Fractionation Kit
产品名称：Mitochondria/Cytosol Fractionation Kit
产品型号：MIT1000
This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells.
merck millipore,默克密理博,MIT1000,Mitochondria/Cytosol Fractionation Kit
- 产品介绍
描述 产品目录编号 MIT1000 品牌系列 Chemicon®
商名 - Chemicon
描述 Mitochondria/Cytosol Fractionation Kit 概述 Mitochondria, sometimes described as the “power plants” of the cell, are sites where most of the energy production in eukaryotic cells takes place. The synthesis of most of the adenosine triphosphate (ATP) occurs in these double membraned organelles that are found in living cells. ATP production by the mitochondria is done by the process of respiration, which uses oxygen to generate energy. This is a very efficient process for using food energy to make ATP. In addition to supplying cellular energy, mitochondria are involved in a range of other processes, such as signaling, cellular differentiation, cell death, as well as the control of the cell cycle and cell growth. Mitochondria also regulate crucial apoptosis signaling pathways. The number of mitochondria in a cell varies widely by organism and tissue type. Many cells have only a few mitochondria, whereas others can contain several thousand.
Millipore’s Mitochondria/Cytosol Fractionation Kit provides reagents for quick and efficient isolation of intact mitochondria from cultured cells. This kit allows mitochondrial isolation by using a convenient table top microcentrifuge, and can be used to separate an enriched mitochondrial fraction (heavy membrane fraction) from cytosolic fraction (light membrane fraction). Such separation is useful for studying apoptosis and signaling pathways between the two fractions, by Western blotting or ELISA.
The kit contains sufficient materials for the analysis of 50 samples.
产品信息 部件 - Anti-GAPDH (CS204254): 25 μg
- Isotonic Mitochondrial Buffer (CS204255): 50 mL
- Mitochondrial Lysis Buffer (CS204257): 5 mL
- Protease inhibitor cocktail (CS204253): 1 mL
- Anti-Bcl2 (05-729-25UG): 25 μg
应用 应用 This Mitochondria/Cytosol Fractionation Kit provides reagents for quick & efficient isolation of intact mitochondria from cultured cells. 生物信息 品种反应性 All 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 All components should be store at -20°C for up to one year from date of receipt. 包装信息 数量 1 kit
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