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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M FC, WB, IHC Rb Diluted Culture Supernatant Monoclonal Antibody 描述 产品目录编号 MABS169 描述 Anti-ERK3 Antibody, clone EP1720Y, Rabbit | MABS169 Alternate Names - protein kinase, mitogen-activated 6
- mitogen-activated protein kinase 6
- Extracellular signal-regulated kinase 3
- MAP kinase 6
- MAP kinase isoform p97
- extracellular signal-regulated kinase, p97
- protein kinase, mitogen-activated 5
背景信息 ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4). 产品信息 格式 Diluted Culture Supernatant 控制 - HeLa cell lysate
演示 Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. 应用 主要应用 - Flow Cytometry
- Western Blotting
- Immunohistochemistry
应用说明 Flow Cytometry Analysis: A 1:45 dilution from a representative lot was used in FC.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ERK3 in human breast carcinoma tissue.生物信息 免疫原品种 Synthetic peptide corresponding to human ERK3 at the N-terminus. 表位 N-terminus 克隆 EP1720Y 宿主 Rabbit 特异性 This antibody recognizes ERK3 at the N-terminus. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Demonstrated to react with Human, Mouse and Rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - Q16659
基因符号 - DKFZp686F03189
- ERK3
- HsT17250
- p97MAPK
- ERK-3
- PRKM6
- MAPK 6
- p97-MAPK
- EC 2.7.11.24
纯化方法 Unpurified UniProt编号 - Q16659
UniProt汇总 FUNCTION: Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle By similarity.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium By similarity.
ENZYME REGULATION: Activated by threonine and tyrosine phosphorylation By similarity.
TISSUE SPECIFICITY Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme By similarity.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected ERK3 in HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. 包装信息 数量 100 µL
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