- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,MABN718,Anti-SMAD5 Antibody, clone 3H9
产品名称：Anti-SMAD5 Antibody, clone 3H9
产品型号：MABN718
Detect Smad using this mouse monoclonal antibody, Anti-SMAD5 Antibody, clone 3H9 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry.
merck millipore,默克密理博,MABN718,Anti-SMAD5 Antibody, clone 3H9
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H FC, WB, IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MABN718 描述 Anti-SMAD5 Antibody, clone 3H9 Alternate Names - SMAD5
- Mothers against decapentaplegic homolog 5
- MAD homolog 5
- Mothers against DPP homolog 5
- SMAD family member 5
背景信息 SMAD5, MAD homolog 5, Mothers against DPP homolog 5, JV5-1, SMAD family member 5 encoded by the gene named SMAD5 or MADH5 is a transcriptional cofactor activated by BMP type 1 receptor kinase and like other SMAD family members SMAD5 belongs to the larger TGFbeta superfamily of signaling modulators. SMAD5 forms complexes with SMAD4 when phosphorylated by BMPR1 and migrates to the nucleus. In the absence of phosphorylation SMAD5 is cytoplasmically localized. SMAD5 levels are regulated via ubiquitin mediated proteolysis facilitated by the E3 ligase SMURF1. SMAD5 is involved in many developmental pathways including in hematopoietic cells where SMAD5 activity is involved in the inhibition of the proliferation of hematopoietic progenitor cells. More recent research has shown SMAD5 to be involved in many other pathways too, for instance homozygous mutants die early in development and display a variable phenotype. Moreover SMAD5 is critical for proper granule cell development in the cerebellum. Finally because SMAD5 belongs to the TGFbeta family whose normal function is usually growth suppression, SMAD5 is often mutated in many cancers and its expression is associated with many forms of cancers in a similar fashion to that of its other family members. EMD-Millipore’s Anti-SMAD5 mouse monoclonal antibody has been tested in western blot on HeLa, SK-N-SH, PC-12 , Jurkat , and K562 cell lysates and in paraffin embedded immunohistochemistry on human brain and lung cancer tissue sections and in fluorescent immunocytochemistry on NTERA-2 cells in culture and in flow cytometry on Jurkat cells in culture. 产品信息 格式 Ascites 控制 - HeLa, SK-N-SH, PC-12, Jurkat, and K562 cell lysates
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Detect Smad using this mouse monoclonal antibody, Anti-SMAD5 Antibody, clone 3H9 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry. 主要应用 - Flow Cytometry
- Western Blotting
- Immunohistochemistry
应用说明 Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected SMAD5 in NTERA-2 cells.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SMAD5 in human brain and lung cancer tissues.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SMAD5 in Jurkat cells.
Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 Purified recombinant fragment of human SMAD5 expressed in E. Coli. 克隆 3H9 宿主 Mouse 同种型 IgG1 品种反应性 RatHuman 抗体类型 Monoclonal Antibody 基因符号 - SMAD5
- Dwfc
- JV5-1
- MADH5
- DKFZp781C1895
- DKFZp781O1323
纯化方法 Unpurified UniProt编号 - Q99717
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa, SK-N-SH, PC-12, Jurkat, and K562 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SMAD5 in HeLa, SK-N-SH, PC-12, Jurkat, and K562 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,1831,L-Ascorbic Acid, Free Acid - CAS 50-81-7 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,AB9906,Anti-QKI-6 Antibody
相关产品
merck millipore,默克密理博,71505,D-Tube™ Dialyzer Mini, MWCO 12-14 kDa
merck millipore,默克密理博,AP1148,Anti-DAD1 Mouse mAb (1F3-A4)
merck millipore,默克密理博,6502,Trypsin, Bovine Pancreas
merck millipore,默克密理博,07-815,Anti-phospho-Raf-1 (Tyr340/Tyr341) Antibody
merck millipore,默克密理博,1831,L-Ascorbic Acid, Free Acid - CAS 50-81-7 - Calbiochem
merck millipore,默克密理博,AB9906,Anti-QKI-6 Antibody
merck millipore,默克密理博,208900,Calpastatin, Human, Recombinant, Domain I - Calbiochem
merck millipore,默克密理博,344037,cFMS Receptor Inhibitor II - CAS 959860-85-6 - Calbiochem
merck millipore,默克密理博,AP124-KC,IgG H&L, GTxMS, AP (KC)
merck millipore,默克密理博,16-244,Anti-phospho-EGFR (Tyr1173) Antibody, clone 9H2, Alexa Fluor 488