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品种反应性 主要应用 宿主 格式 抗体类型 H WB, IHC, ELISA, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MABF96 描述 Anti-TRAcP Antibody, clone 9C5 Alternate Names - Type 5 acid phosphatase
- Tartrate-resistant acid phosphatase type 5
- TR-AP
- Tartrate-resistant acid ATPase
- TrATPase
背景信息 Tartrate-resistant acid phosphatase type 5 (TRAcP), also referred to as Acp5, is secreted by osteoclasts during bone resorption. Release of TRAcP was stimulated by resorbogenic cytokines and repressed by calcitonin (a resorption inhibitor). TRAcP is also known to be expressed in high quantities in active macrophages and dendritic cells and play an essential part in presenting antigens to T cells. Inactive TRAcP released from adipose tissue macrophages has been shown to bring on hyperplastic obesity, keeping lipid metabolism and insulin sensitivity normal. 产品信息 格式 Purified 控制 - Large malignant B-cells in hairy cell leukemia tissue
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-TRAcP Antibody, clone 9C5 detects level of TRAcP & has been published & validated for use in IH, IP, DB, ELISA, IC & WB. 主要应用 - Western Blotting
- Immunohistochemistry
- ELISA
- Immunocytochemistry
应用说明 Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)
Dot Blot Analysis: A representative lot was used by an independent laboratory in DB. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)
ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Janckila, A.J., et al. (1995). Blood. 85:2839-2844.)
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Janckila, A.J., et al. (1996). The Journal of Histochemistry and Cytochemistry. 44(3):233-244.)生物信息 免疫原品种 Full length purified protein corresponding to human TRAcP. 克隆 9C5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgG2bκ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001104504
Entrez基因汇总 This gene encodes an iron containing glycoprotein which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. [provided by RefSeq]. 基因符号 - ACP5
纯化方法 Protein G UniProt编号 - P13686
UniProt汇总 FUNCTION: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias.
CATALYTIC ACTIVITY: A phosphate monoester + H2O = an alcohol + phosphate.
COFACTOR: Binds 2 iron ions per subunit.
SUBUNIT STRUCTURE: Exists either as monomer or, after proteolytic processing, as a dimer of two chains linked by disulfide bond(s).
SUBCELLULAR LOCATION: Lysosome.
SEQUENCE SIMILARITIES: Belongs to the metallophosphoesterase superfamily. Purple acid phosphatase family.产品使用声明 质量保证 Evaluated by Immunohistochemistry in large malignant B-cells in hairy cell leukemia tissue.
Immunohistochemistry Analysis: 1:500 dilution of this antibody detected TRAcP in large malignant B-cells in hairy cell leukemia tissue.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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