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merck millipore,默克密理博,MABE953,Anti-phospho RNA Pol II (Ser2), clone 3E7C7 Antibody
产品名称：Anti-phospho RNA Pol II (Ser2), clone 3E7C7 Antibody
产品型号：MABE953
This Anti-phospho RNA Pol II (Ser2) antibody, clone 3E7C7 is validated for use in western blotting, ChIP-seq, ELISA, ICC & ChIP for the detection of phospho RNA Pol II (Ser2).
merck millipore,默克密理博,MABE953,Anti-phospho RNA Pol II (Ser2), clone 3E7C7 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, Mk, Ca, M ChIP, WB, ICC R Purified Monoclonal Antibody 描述 产品目录编号 MABE953 描述 Anti-phospho RNA Pol II (Ser2), clone 3E7C7 Antibody Alternate Names - RNA-directed RNA polymerase II subunit RPB1
- DNA-directed RNA polymerase II subunit RPB1
- RNA polymerase II subunit B1
- DNA-directed RNA polymerase II subunit A
- DNA-directed RNA polymerase III largest subunit
背景信息 RNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription. As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity. 产品信息 格式 Purified 演示 Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-phospho RNA Pol II (Ser2) antibody, clone 3E7C7 is validated for use in western blotting, ChIP-seq, ELISA, ICC & ChIP for the detection of phospho RNA Pol II (Ser2). 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
- Immunocytochemistry
应用说明 Western Blotting Analysis: 0.5 µg/mL from a representative lot detected phospho RNA Pol II (Ser2) in 10 µg of HeLa nuclear extract.
Western Blotting: A representative lot detected phospho RNA Pol II (Ser2) in 10 µg of HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.).
Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho RNA Pol II (Ser2) in HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.; Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).
Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated phospho RNA Pol II (Ser2) in HeLa cell lysate (Odawara, J., et al. (2011). BMC Genomics. 12:516.; Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.).
ELISA Analysis: A representative lot from an independent laboratory detected phospho RNA Pol II (Ser2) in a panel of unmodified and modified RNA Pol II (Ser2) proteins (Prof. Taro Tachibana, Cell Engineering Corporation.).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot from an independent laboratory detected phospho RNA Pol II (Ser2) in HeLa cells (Prof. Taro Tachibana, Cell Engineering Corporation.).
Alexa Fluor® is a registered trademark of Life Technologies®.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human RNA Pol II phosphorylated at Ser2. 克隆 3E7C7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 同种型 IgG2aκ 品种反应性 RatHumanMonkeyCanineMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000928
基因符号 - POLR2
- POLR2A
纯化方法 Protein G purified UniProt编号 - P24928
产品使用声明 质量保证 Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho RNA Pol II (Ser2) in 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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