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merck millipore,默克密理博,HTS206M,ChemiSCREEN™ Membrane Preparation Recombinant Human MrgD Mas-Related Gene Family Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human MrgD Mas-Related Gene Family Receptor
产品型号：HTS206M
Human MRGPRD / MrgD GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS206M,ChemiSCREEN™ Membrane Preparation Recombinant Human MrgD Mas-Related Gene Family Receptor
- 产品介绍
- merck millipore,默克密理博,HTS206M,ChemiSCREEN™ Membrane Preparation Recombinant Human MrgD Mas-Related Gene Family Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 MRGPRD / MrgD 描述 产品目录编号 HTS206M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human MrgD Mas-Related Gene Family Receptor 概述 Full-length human MRGPRD cDNA encoding MrgD 背景信息 The Mas-related genes (Mrgs), also known as sensory neuron-specific G-protein coupled receptors (SNSRs), are selectively expressed in subpopulations of sensory neurons involved in the perceptor of pain (Dong et al., 2001). The Mrg family in rodents consists of 50 receptors in the MrgA-MrgH subfamilies, but humans contain fewer Mrg members. MrgD (also known as TGR7) is found across species from rodents to humans, and its expression is restricted to nonpeptidergic, small-diameter, IB4+ dorsal root ganglion (DRG) neurons. MrgD binds β-alanine, but not L-alanine, which results in increased intracellular calcium and the suppression of forskolin-induced cAMP production, thus suggesting coupling to Gq and Gi proteins (Shinohara et al., 2004). β-alanine-induced activation of MrgD inhibits KCNQ2/3-mediated M-currents in cells overexpressing MrgD and KCNQ2/3 and also in cultured DRG neurons, which indicates that MrgD enhances nociceptive signaling (Crozier et al., 2007). Millipore's MrgD membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of MrgD interactions with its ligands. The cell line exhibits a calcium response with EC50s of 11 µM for β-alanine. The membrane preparations exhibit EC50s of 69 µM for β-alanine in a GTPS binding assay. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C应用 应用 Human MRGPRD / MrgD GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_198923.2
基因符号 - TGR7
- MRGPRD
- MRGD
- mrgD
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 MRGPRD / MrgD 靶子系列 Mas-related gene UniProt编号 - Q8TDS7
UniProt汇总 FUNCTION: SwissProt: Q8TDS7 # May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. Beta-alanine at micromolar doses specifically evoked Ca(2+) influx in cells expressing the receptor. Beta-alanine decreases forskolin- stimulated cAMP production in cells expressing the receptor, suggesting that the receptor couples with G-protein G(q) and G(i).
SIZE: 321 amino acids; 36118 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane but internalized into the cytoplasm after treatment with beta-alanine.
SIMILARITY: SwissProt: Q8TDS7 ## Belongs to the G-protein coupled receptor 1 family. Mas subfamily.孵化条件 ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [35S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl2/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled β-alanine was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to a GF/B filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.
One vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific β-alanine -stimulated [35S]-GTPγS binding.
The MrgD membrane preparation is expected to be functional in a radioligand binding assay; however, the end user will need to determine the optimal radiolabeled ligand for use with this product.产品使用声明 质量保证 SPECIFICATIONS: 1 unit = 5 µg membrane preparation
EC50 in GTPγS binding assay by β-alanine: ~ 69 μM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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