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merck millipore,默克密理博,MABE8,Anti-EBNA2 Antibody, clone R3
产品名称：Anti-EBNA2 Antibody, clone R3
产品型号：MABE8
Anti-EBNA2 Antibody, clone R3 is a Rat Monoclonal Antibody for detection of EBNA2 also known as Epstein-Barr nuclear antigen 2 & has been validated in WB, IP & IHC.
merck millipore,默克密理博,MABE8,Anti-EBNA2 Antibody, clone R3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, WB R Purified Monoclonal Antibody 描述 产品目录编号 MABE8 描述 Anti-EBNA2 Antibody, clone R3 概述 Gross, H., et al. (2010). Virology. 397(2): 299-310.
PMID: 19969318
Kremmer, E., et al. (1995). Virology. 208(1): 336-342.
PMID: 11831716Alternate Names - Epstein-Barr nuclear antigen 2
背景信息 The Epstein-Barr virus or EBV is associated with several human cancers and actively immortalizes B lymphocytes. EBV is leads to expression by a small group of viral proteins, including Epstein-Barr nuclear antigen 2 (EBNA2) which is required for immortalization. EBNA2 is a transcriptional activator belonging to the herpesviridae EBNA2 family, and performs a crucial role in activating the host resting B-cell and inducing B-cell proliferation. EBNA2 possesses an acidic activating domain with which it is able to recruit various cellular and viral involved in B-cell immortalization. Implicated in cellular phenotypic modifications, EBNA2 is known to upregulate the expression of cell surface receptors, such as CD21, MYC, and CD23, as well as upregulating viral genes such as LMP1, LMP2A, LMP2B, and EBNA1-6. 产品信息 格式 Purified 控制 - B95-8 cell lysate
演示 Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-EBNA2 Antibody, clone R3 is a Rat Monoclonal Antibody for detection of EBNA2 also known as Epstein-Barr nuclear antigen 2 & has been validated in WB, IP & IHC. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Gross, H., et al. (2010). Virology. 397(2):299-310.)
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Kremmer, E., et al. (1995). Virology. 208(1):336-342.)生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human EBNA2. 表位 C-terminus of EBNA2 克隆 R3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 同种型 IgG2aκ 品种反应性 Human Species Reactivity Note Demonstrated to react with human cell infected with EBV. 抗体类型 Monoclonal Antibody Entrez基因编号 - YP_001129441
基因符号 - EBNA2
纯化方法 Protein G UniProt编号 - Q69022
UniProt汇总 FUNCTION: Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by upregulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells (By similarity).
SUBUNIT STRUCTURE: Interacts with human SMARCB1/INI1, presumably generating an open chromatin conformation at the EBNA2-responsive target genes. Interacts with human WAPAL. Interacts with host CBF1; this interaction allows transcriptional activation by EBNA2. Interacts with host general transcription factors GTF2B, ERCC2 and ERCC3 (By similarity).
SUBCELLULAR LOCATION: Host nucleus matrix. Note: Associated with the nuclear matrix (By similarity).
PTM: Phosphorylated (By similarity).
SEQUENCE SIMILARITIES: Belongs to the herpesviridae EBNA2 family.产品使用声明 质量保证 Evaluated by Western Blot in B95-8 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected EBNA2 on 10 µg of B95-8 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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