merck millipore,默克密理博,MABE453,Anti-monoubiquityl Histone H2B (Lys119) Antibody, clone 7B4

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
H	IHC, WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	MABE453
描述	Anti-monoubiquityl Histone H2B (Lys119) Antibody, clone 7B4
Alternate Names	H2BK119Ub H2BUb1 Histone H2B type 1-C/E/F/G/I Histone H2B.1 A Histone H2B.a H2B/a Histone H2B.g H2B/g Histone H2B.h H2B/h Histone H2B.k H2B/k Histone H2B.l H2B/l H2BUbK119
背景信息	H2BUb1, also known as Histone H2B.1 A, or Histone H2B.a (H2B/a), or Histone H2B.g (H2B/g), or Histone H2B.h (H2B/h), or Histone H2B.k (H2B/k) or Histone H2B.i (H2B/I;H2B/I), and encoded by the gene HIST1H2BC or HIST1H2BE, HIST1H2BF, HIST1H2BG, HIST1H2BI/H2BFL, H2BFH, H2BFG, H2BFA, H2BFK, is a core component of the nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H2BUb1 also has broad antibacterial activity and may contribute to the formation of the functional antimicrobial barrier of the colonic epithelium, and to the bactericidal activity of amniotic fluid. H2BUb1 is localized to the nucleus and chromosomes in healthy cells.

产品信息
格式	Purified
演示	Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	Detect Histone H2B using this mouse monoclonal antibody, Anti-monoubiquityl Histone H2B (Lys119) Antibody, clone 7B4 validated for use in western blotting & IHC.
主要应用	Immunohistochemistry Western Blotting
应用说明	Western Blotting Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in hormone deprived MCF7 cell lysates, which were pretreated with vehicle (ethanol) or Bortezomid before incubating with 17Beta-Estradiol (Prenzel, T., et al. (2011). AACR. OF1-OF15). Western Blotting Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in differentiated hMSCs that were truncated with control and demostrated a loss of signal in differntiated hMSCs that ere transfected with RNF50 siRNA (Karpiuk, O., et al. (2012). Molecular Cell. 46:705-713). Immunohistochemistry Analysis: A representative lot detected monoubiquityl Histone H2B (Lys119) in malignant, metastatic breast tumor and normal adjacent tissues (Prenzel, T., et al. (2011). AACR. OF1-OF15).

生物信息
免疫原品种	Ovalbumin-conjugated corresponding to human monoubiquityl Histone H2B (Lys119).
克隆	7B4
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
同种型	IgG2aκ
品种反应性	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_003509
基因符号	H2BFK HIST1H2BC H2BFL HIST1H2BE H2BFH HIST1H2BF H2BFG HIST1H2BG H2BFA HIST1H2BI
纯化方法	Protein G purified
UniProt编号	P62807

产品使用声明
质量保证	Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected monoubiquityl Histone H2B (Lys119) in HeLa cell lysates treated with and without Nocodazole.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	100 µg

merck millipore,默克密理博,MABE453,Anti-monoubiquityl Histone H2B (Lys119) Antibody, clone 7B4

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