merck millipore,默克密理愽
  • merck millipore,默克密理博,MABE308,Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10

  • merck millipore,默克密理博,MABE308,Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10

    产品名称：Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10
    产品型号：MABE308
    Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10 is a Mouse Monoclonal Antibody for detection of ICBP90/UHRF1 also known as E3 ubiquitin-protein ligase, inverted CCAAT box-binding protein & has been validat More>>

    merck millipore,默克密理博,MABE308,Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10

  • 产品介绍
  • merck millipore,默克密理博,MABE308,Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HIHC, WB, ChIP, ICCMAscitesMonoclonal Antibody
    描述
    产品目录编号 MABE308
    描述 Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10
    Alternate Names
    • and RING finger domains protein 1
    • E3 ubiquitin-protein ligase
    • inverted CCAAT box-binding protein
    • Nuclear protein 95
    • Nuclear zinc finger protein Np95
    • HuNp95
    • RING finger protein 106
    • Transcription factor ICBP90
    • Ubiquitin-like PHD and RING finger domain-containing protein 1
    • Ubiquitin-like-containing PHD
    背景信息 Ubiquitin-like with PHD and ring finger domains 1 (UHRF1), also known as ICBP90 or Np95, is a multifunctional nuclear protein that may be an important mediator of heterochromatin formation which is indicative of gene repression. UHRF1 contains a set and RING-finger-associated domain that detects hemi-methylated cytosine residues leading to the recruitment of DNMT1 to these residues during the S phase. UHRF1 thereby facilitates the transfer of methylation status to newly synthesized DNA molecules. UHRF1 may also interact with histones and HDAC1 via its PHD domain to promote modifications of histone residues which contribute to heterochromatin formation. UHRF1 may also directly modify histone H3 by ubiquitination. In addition, UHRF1 may contribute to the DNA-repair process though associations with PCNA. Several studies have suggested that overexpression of UHRF1 may contribute to the development of many cancers.
    产品信息
    格式 Ascites
    控制
    • HeLa cell lysate
    演示 Mouse monoclonal IgG1 ascites with 0.05% sodium azide.
    应用
    应用 Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10 is a Mouse Monoclonal Antibody for detection of ICBP90/UHRF1 also known as E3 ubiquitin-protein ligase, inverted CCAAT box-binding protein & has been validated in WB, ChIP, ICC & IHC.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    • Chromatin Immunoprecipitation (ChIP)
    • Immunocytochemistry
    应用说明 Western Blot Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in HeLa and MOLT-4 cell lysates (Hopfner, R., et al. (2001). 266(1-2)15-23.).

    Immunocytochemistry Analyisis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in ICC (Hopfner, R., et al. (2001). Gene. 266(1-2)15-23.).

    Immunohistochemistry Analysis: A representative lot from an independent laboratory detected ICBP90/UHRF1 in human appendix and human breast carcinoma tissues (Hopfner, R., et al. (2000). Cancer Res. 60(1):121-128.).

    Chromatin Immunoprecipitation Analyis: A representative lot from an independent laboratory immunoprecipitated ICB90/UHRF1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19). 8355-8365.)
    生物信息
    免疫原品种 Recombinant protein corresponding to human ICBP90.
    克隆 1RC-1C10
    宿主 Mouse
    同种型 IgG1
    品种反应性 Human
    Species Reactivity Note Demonstrated to react with Human.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_001041666
    Entrez基因汇总 This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.
    基因符号
    • UHRF1
    • ICBP90
    • NP95
    • RNF106
    纯化方法 Unpurified
    UniProt编号
    • Q96T88
    UniProt汇总 FUNCTION: Putative E3 ubiquitin-protein ligase. May participate in methylation-dependent transcriptional regulation. Binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. Important for G1/S transition. May be involved in DNA repair and chromosomal stability.

    PATHWAY: Protein modification; protein ubiquitination.

    SUBUNIT STRUCTURE: Interacts with histones H3, H1 and H2B. Interacts with HDAC1, but not with HDAC2. Interacts with UHRF1BP1. Binds methylated CpG containing oligonucleotides.

    SUBCELLULAR LOCATION: Nucleus.

    TISSUE SPECIFICITY: Expressed in thymus, bone marrow, testis, lung and heart. Overexpressed in breast cancer.

    DEVELOPMENTAL STAGE. Expressed in fetal thymus, liver and kidney.

    INDUCTION: Up-regulated in proliferating cells, and down-regulated in quiescent cells. Down-regulated upon adriamycin-induced DNA damage, in a p53/TP53 and CDKN1A-dependent way. Induced by E2F1 transcription factor.

    DOMAIN:The RING finger is required for ubiquitin ligase activity. The YDG domain mediates the interaction with histone H3

    POST-TRANSLATIONAL MODIFICATION: Phosphorylated on serine residues. Phosphorylation may enhance DNA-binding activity. Ubiquitinated; which leads to proteasomal degradation. Polyubiquitination may be stimulated by DNA damage.

    SEQUENCE SIMILARITIES: Contains 1 PHD-type zinc finger. Contains 2 RING-type zinc fingers. Contains 1 ubiquitin-like domain. Contains 1 YDG domain.

    SEQUENCE CAUTION: The sequence BAB15177.1 differs from that shown. Reason: Erroneous initiation.
    产品使用声明
    质量保证 Evaluated by Western Blot in HeLa cell lysate.

    Western Blot Analysis: A 1:5,000 dilution of this antibody detected ICBP90/UHRF1 in 10 µg of HeLa cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.
    Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MABE308,Anti-ICBP90/UHRF1 Antibody, clone 1RC1C-10

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